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@ARTICLE{Viennet:856614,
author = {Viennet, Thibault and Wördehoff, Michael M. and Uluca,
Boran and Poojari, Chetan and Shaykhalishahi, Hamed and
Willbold, Dieter and Strodel, Birgit and Heise, Henrike and
Buell, Alexander K. and Hoyer, Wolfgang and Etzkorn, Manuel},
title = {{S}tructural insights from lipid-bilayer nanodiscs link
α-{S}ynuclein membrane-binding modes to amyloid fibril
formation},
journal = {Communications biology},
volume = {1},
number = {1},
issn = {2399-3642},
address = {London},
publisher = {Springer Nature},
reportid = {FZJ-2018-05981},
pages = {44},
year = {2018},
abstract = {The protein α-Synuclein (αS) is linked to Parkinson’s
disease through its abnormal aggregation, which is thought
to involve cytosolic and membrane-bound forms of αS.
Following previous studies using micelles and vesicles, we
present a comprehensive study of αS interaction with
phospholipid bilayer nanodiscs. Using a combination of
NMR-spectroscopic, biophysical, and computational methods,
we structurally and kinetically characterize αS interaction
with different membrane discs in a quantitative and
site-resolved way. We obtain global and residue-specific αS
membrane affinities, and determine modulations of αS
membrane binding due to αS acetylation, membrane
plasticity, lipid charge density, and accessible membrane
surface area, as well as the consequences of the different
binding modes for αS amyloid fibril formation. Our results
establish a structural and kinetic link between the observed
dissimilar binding modes and either aggregation-inhibiting
properties, largely unperturbed aggregation, or accelerated
aggregation due to membrane-assisted fibril nucleation.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30271927},
UT = {WOS:000461126500044},
doi = {10.1038/s42003-018-0049-z},
url = {https://juser.fz-juelich.de/record/856614},
}