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@ARTICLE{Siebers:856637,
author = {Siebers, M. and Rohr, T. and Ventura, M. and Thies, Stephan
and Kovacic, Filip and Jaeger, Karl-Erich and Berg, M. and
Doermann, P. and Schulz, M.},
title = {{D}isruption of microbial community composition and
identification of plant growth promoting microorganisms
after exposure of soil to rapeseed-derived glucosinolates},
journal = {PLOS ONE},
volume = {13},
issn = {1932-6203},
address = {San Francisco, California, US},
publisher = {PLOS},
reportid = {FZJ-2018-06002},
pages = {13:e0200160},
year = {2018},
abstract = {Land plants are engaged in intricate communities with soil
bacteria and fungi indispensable for plant survival and
growth. The plant-microbial interactions are largely
governed by specific metabolites. We employed a combination
of lipid-fingerprinting, enzyme activity assays,
high-throughput DNA sequencing and isolation of cultivable
microorganisms to uncover the dynamics of the bacterial and
fungal community structures in the soil after exposure to
isothiocyanates (ITC) obtained from rapeseed glucosinolates.
Rapeseed-derived ITCs, including the cyclic, stable goitrin,
are secondary metabolites with strong allelopathic affects
against other plants, fungi and nematodes, and in addition
can represent a health risk for human and animals. However,
the effects of ITC application on the different bacterial
and fungal organisms in soil are not known in detail. ITCs
diminished the diversity of bacteria and fungi. After
exposure, only few bacterial taxa of the
Gammaproteobacteria, Bacteriodetes and Acidobacteria
proliferated while Trichosporon (Zygomycota) dominated the
fungal soil community. Many surviving microorganisms in
ITC-treated soil where previously shown to harbor plant
growth promoting properties. Cultivable fungi and bacteria
were isolated from treated soils. A large number of
cultivable microbial strains was capable of mobilizing
soluble phosphate from insoluble calcium phosphate, and
their application to Arabidopsis plants resulted in
increased biomass production, thus revealing growth
promoting activities. Therefore, inclusion of
rapeseed-derived glucosinolates during biofumigation causes
losses of microbiota, but also results in enrichment with
ITC-tolerant plant microorganisms, a number of which show
growth promoting activities, suggesting that Brassicaceae
plants can shape soil microbiota community structure
favoring bacteria and fungi beneficial for Brassica plants.},
cin = {IMET},
ddc = {610},
cid = {I:(DE-Juel1)IMET-20090612},
pnm = {581 - Biotechnology (POF3-581)},
pid = {G:(DE-HGF)POF3-581},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29969500},
UT = {WOS:000437246700050},
doi = {10.1371/journal.pone.0200160},
url = {https://juser.fz-juelich.de/record/856637},
}