%0 Journal Article
%A Dang, Ka My
%A Rinklin, Philipp
%A Afanasenkau, Dzmitry
%A Westmeyer, Gil
%A Schürholz, Tobias
%A Wiegand, Simone
%A Wolfrum, Bernhard
%T Chip-Based Heat Stimulation for Modulating Signal Propagation in HL-1 Cell Networks
%J Advanced biosystems
%V 2
%@ 2366-7478
%C Weinheim
%I Wiley-VCH
%M FZJ-2018-06216
%P 1800138 -
%D 2018
%X Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, we investigate the response of a connected HL-1 cardiomyocyte cell network to the application of confined heat stimuli using Ca2+ imaging. Localized temperature gradients are generated by resistive heating via microwire arrays on a chip surface, which serves as a substrate for growing a confluent cell network. We demonstrate that upon heat stimulation, the velocity of the propagating Ca2+ wave in the network is locally increased, leading to a deformation of the wavefront. Furthermore, we show evidence of a change in the signal propagation direction caused by a relocation of the pacemaker cell. This effect might be used in future applications, where heat is employed as an alternative modality for cell stimulation protocols.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000452532700006
%R 10.1002/adbi.201800138
%U https://juser.fz-juelich.de/record/856884