TY  - JOUR
AU  - Dang, Ka My
AU  - Rinklin, Philipp
AU  - Afanasenkau, Dzmitry
AU  - Westmeyer, Gil
AU  - Schürholz, Tobias
AU  - Wiegand, Simone
AU  - Wolfrum, Bernhard
TI  - Chip-Based Heat Stimulation for Modulating Signal Propagation in HL-1 Cell Networks
JO  - Advanced biosystems
VL  - 2
SN  - 2366-7478
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2018-06216
SP  - 1800138 -
PY  - 2018
AB  - Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, we investigate the response of a connected HL-1 cardiomyocyte cell network to the application of confined heat stimuli using Ca2+ imaging. Localized temperature gradients are generated by resistive heating via microwire arrays on a chip surface, which serves as a substrate for growing a confluent cell network. We demonstrate that upon heat stimulation, the velocity of the propagating Ca2+ wave in the network is locally increased, leading to a deformation of the wavefront. Furthermore, we show evidence of a change in the signal propagation direction caused by a relocation of the pacemaker cell. This effect might be used in future applications, where heat is employed as an alternative modality for cell stimulation protocols.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000452532700006
DO  - DOI:10.1002/adbi.201800138
UR  - https://juser.fz-juelich.de/record/856884
ER  -