TY  - JOUR
AU  - Halbwachs, Hans
AU  - Easton, Gary L.
AU  - Bol, Roland
AU  - Hobbie, Erik A.
AU  - Garnett, Mark H
AU  - Peršoh, Derek
AU  - Dixon, Liz
AU  - Ostle, Nick
AU  - Karasch, Peter
AU  - Griffith, Gareth W.
TI  - Isotopic evidence of biotrophy and unusual nitrogen nutrition in soil-dwelling Hygrophoraceae
JO  - Environmental microbiology
VL  - 20
IS  - 10
SN  - 1462-2912
CY  - Oxford [u.a.]
PB  - Blackwell
M1  - FZJ-2018-06371
SP  - 3573 - 3588
PY  - 2018
AB  - Several lines of evidence suggest that the agaricoid, non‐ectomycorrhizal members of the family Hygrophoraceae (waxcaps) are biotrophic with unusual nitrogen nutrition. However, methods for the axenic culture and lab‐based study of these organisms remain to be developed, so our current knowledge is limited to field‐based investigations. Addition of nitrogen, lime or organophosphate pesticide at an experimental field site (Sourhope) suppressed fruiting of waxcap basidiocarps. Furthermore, stable isotope natural abundance in basidiocarps were unusually high in 15N and low in 13C, the latter consistent with mycorrhizal nutritional status. Similar patterns were found in waxcap basidiocarps from diverse habitats across four continents. Additional data from 14C analysis of basidiocarps and 13C pulse label experiments suggest that these fungi are not saprotrophs but rather biotrophic endophytes and possibly mycorrhizal. The consistently high but variable δ15N values (10–20‰) of basidiocarps further indicate that N acquisition or processing differ from other fungi; we suggest that N may be derived from acquisition of N via soil fauna high in the food chain. 
LB  - PUB:(DE-HGF)16
C6  - pmid:30105856
UR  - <Go to ISI:>//WOS:000447549700009
DO  - DOI:10.1111/1462-2920.14327
UR  - https://juser.fz-juelich.de/record/857127
ER  -