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@ARTICLE{Btepage:857531,
author = {Bütepage, Mareike and Preisinger, Christian and von
Kriegsheim, Alexander and Scheufen, Anja and Lausberg, Eva
and Li, Jinyu and Kappes, Ferdinand and Feederle, Regina and
Ernst, Sabrina and Eckei, Laura and Krieg, Sarah and
Müller-Newen, Gerhard and Rossetti, Giulia and Feijs, Karla
L. H. and Verheugd, Patricia and Lüscher, Bernhard},
title = {{N}ucleolar-nucleoplasmic shuttling of {TARG}1 and its
control by {DNA} damage-induced poly-{ADP}-ribosylation and
by nucleolar transcription},
journal = {Scientific reports},
volume = {8},
number = {1},
issn = {2045-2322},
address = {[London]},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2018-06522},
pages = {6748},
year = {2018},
abstract = {Macrodomains are conserved protein folds associated with
ADP-ribose binding and turnover. ADP-ribosylation is a
posttranslational modification catalyzed primarily by ARTD
(aka PARP) enzymes in cells. ARTDs transfer either single or
multiple ADP-ribose units to substrates, resulting in mono-
or poly-ADP-ribosylation. TARG1/C6orf130 is a macrodomain
protein that hydrolyzes mono-ADP-ribosylation and interacts
with poly-ADP-ribose chains. Interactome analyses revealed
that TARG1 binds strongly to ribosomes and proteins
associated with rRNA processing and ribosomal assembly
factors. TARG1 localized to transcriptionally active
nucleoli, which occurred independently of ADP-ribose
binding. TARG1 shuttled continuously between nucleoli and
nucleoplasm. In response to DNA damage, which activates
ARTD1/2 (PARP1/2) and promotes synthesis of poly-ADP-ribose
chains, TARG1 re-localized to the nucleoplasm. This was
dependent on the ability of TARG1 to bind to
poly-ADP-ribose. These findings are consistent with the
observed ability of TARG1 to competitively interact with RNA
and PAR chains. We propose a nucleolar role of TARG1 in
ribosome assembly or quality control that is stalled when
TARG1 is re-located to sites of DNA damage.},
cin = {IAS-5 / JSC},
ddc = {600},
cid = {I:(DE-Juel1)IAS-5-20120330 / I:(DE-Juel1)JSC-20090406},
pnm = {574 - Theory, modelling and simulation (POF3-574) / 511 -
Computational Science and Mathematical Methods (POF3-511)},
pid = {G:(DE-HGF)POF3-574 / G:(DE-HGF)POF3-511},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29712969},
UT = {WOS:000431107100007},
doi = {10.1038/s41598-018-25137-w},
url = {https://juser.fz-juelich.de/record/857531},
}