TY  - JOUR
AU  - Wördehoff, Michael
AU  - Hoyer, Wolfgang
TI  - α-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay
JO  - Bio-protocol
VL  - 8
IS  - 14
SN  - 2331-8325
CY  - Sunnyvale, CA
PB  - bio-protocol.org
M1  - FZJ-2018-07290
SP  - PMC6066150
PY  - 2018
AB  - Studying the aggregation of amyloid proteins like α-synuclein in vitro is a convenient and popular tool to gain kinetic insights into aggregation as well as to study factors (e.g., aggregation inhibitors) that influence it. These aggregation assays typically make use of the fluorescence dye Thioflavin T as a sensitive fluorescence reporter of amyloid fibril formation and are conducted in a plate-reader-based format, permitting the simultaneous screening of multiple samples and conditions. However, aggregation assays are generally prone to poor reproducibility due to the stochastic nature of fibril nucleation and the multiplicity of modulating factors. Here we present a simple and reproducible protocol to study the aggregation of α-synuclein in a plate-reader based assay.
LB  - PUB:(DE-HGF)16
C6  - pmid:30069495
UR  - <Go to ISI:>//WOS:000457966200014
DO  - DOI:10.21769/BioProtoc.2941
UR  - https://juser.fz-juelich.de/record/858404
ER  -