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@ARTICLE{Wrdehoff:858404,
      author       = {Wördehoff, Michael and Hoyer, Wolfgang},
      title        = {α-{S}ynuclein {A}ggregation {M}onitored by {T}hioflavin
                      {T} {F}luorescence {A}ssay},
      journal      = {Bio-protocol},
      volume       = {8},
      number       = {14},
      issn         = {2331-8325},
      address      = {Sunnyvale, CA},
      publisher    = {bio-protocol.org},
      reportid     = {FZJ-2018-07290},
      pages        = {PMC6066150},
      year         = {2018},
      abstract     = {Studying the aggregation of amyloid proteins like
                      α-synuclein in vitro is a convenient and popular tool to
                      gain kinetic insights into aggregation as well as to study
                      factors (e.g., aggregation inhibitors) that influence it.
                      These aggregation assays typically make use of the
                      fluorescence dye Thioflavin T as a sensitive fluorescence
                      reporter of amyloid fibril formation and are conducted in a
                      plate-reader-based format, permitting the simultaneous
                      screening of multiple samples and conditions. However,
                      aggregation assays are generally prone to poor
                      reproducibility due to the stochastic nature of fibril
                      nucleation and the multiplicity of modulating factors. Here
                      we present a simple and reproducible protocol to study the
                      aggregation of α-synuclein in a plate-reader based assay.},
      cin          = {ICS-6},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30069495},
      UT           = {WOS:000457966200014},
      doi          = {10.21769/BioProtoc.2941},
      url          = {https://juser.fz-juelich.de/record/858404},
}