% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Beckers:858495,
      author       = {Beckers, Maximilian and Jakobi, Arjen J. and Sachse,
                      Carsten},
      title        = {{T}hresholding of cryo-{EM} density maps by false discovery
                      rate control},
      journal      = {IUCrJ},
      volume       = {6},
      number       = {1},
      issn         = {2052-2525},
      address      = {Chester},
      reportid     = {FZJ-2018-07367},
      pages        = {1-16},
      year         = {2019},
      abstract     = {Cryo-EM now commonly generates close-to-atomic resolution
                      as well as intermediate resolution maps from macromolecules
                      observed in isolation and in situ. Interpreting these maps
                      remains a challenging task owing to poor signal in the
                      highest resolution shells and the necessity to select a
                      threshold for density analysis. In order to facilitate this
                      process, a statistical framework for the generation of
                      confidence maps by multiple hypothesis testing and false
                      discovery rate (FDR) control has been developed. In this
                      way, three-dimensional confidence maps contain signal
                      separated from background noise in the form of local
                      detection rates of EM density values. It is demonstrated
                      that confidence maps and FDR-based thresholding can be used
                      for the interpretation of near-atomic resolution
                      single-particle structures as well as lower resolution maps
                      determined by subtomogram averaging. Confidence maps
                      represent a conservative way of interpreting molecular
                      structures owing to minimized noise. At the same time they
                      provide a detection error with respect to background noise,
                      which is associated with the density and is particularly
                      beneficial for the interpretation of weaker cryo-EM
                      densities in cases of conformational flexibility and lower
                      occupancy of bound molecules and ions in the structure.},
      cin          = {ER-C-3},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000455767600004},
      pubmed       = {pmid:30713700},
      doi          = {10.1107/S2052252518014434},
      url          = {https://juser.fz-juelich.de/record/858495},
}