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| Hauptseite > Publikationsdatenbank > Thresholding of cryo-EM density maps by false discovery rate control > print |
| Hauptseite > Publikationsdatenbank > Thresholding of cryo-EM density maps by false discovery rate control > print |
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| 100 | 1 | _ | |a Beckers, Maximilian |0 0000-0001-5988-1322 |b 0 |
| 245 | _ | _ | |a Thresholding of cryo-EM density maps by false discovery rate control |
| 260 | _ | _ | |a Chester |c 2019 |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Cryo-EM now commonly generates close-to-atomic resolution as well as intermediate resolution maps from macromolecules observed in isolation and in situ. Interpreting these maps remains a challenging task owing to poor signal in the highest resolution shells and the necessity to select a threshold for density analysis. In order to facilitate this process, a statistical framework for the generation of confidence maps by multiple hypothesis testing and false discovery rate (FDR) control has been developed. In this way, three-dimensional confidence maps contain signal separated from background noise in the form of local detection rates of EM density values. It is demonstrated that confidence maps and FDR-based thresholding can be used for the interpretation of near-atomic resolution single-particle structures as well as lower resolution maps determined by subtomogram averaging. Confidence maps represent a conservative way of interpreting molecular structures owing to minimized noise. At the same time they provide a detection error with respect to background noise, which is associated with the density and is particularly beneficial for the interpretation of weaker cryo-EM densities in cases of conformational flexibility and lower occupancy of bound molecules and ions in the structure. |
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| 700 | 1 | _ | |a Jakobi, Arjen J. |0 0000-0002-7761-2027 |b 1 |
| 700 | 1 | _ | |a Sachse, Carsten |0 P:(DE-Juel1)173949 |b 2 |e Corresponding author |
| 773 | _ | _ | |a 10.1107/S2052252518014434 |g Vol. 6, no. 1 |0 PERI:(DE-600)2754953-7 |n 1 |p 1-16 |t IUCrJ |v 6 |y 2019 |x 2052-2525 |
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