000858501 001__ 858501
000858501 005__ 20210130000003.0
000858501 0247_ $$2doi$$a10.3389/fmicb.2018.03033
000858501 0247_ $$2Handle$$a2128/20763
000858501 0247_ $$2WOS$$aWOS:000452616600001
000858501 0247_ $$2altmetric$$aaltmetric:52445000
000858501 037__ $$aFZJ-2018-07372
000858501 082__ $$a570
000858501 1001_ $$0P:(DE-HGF)0$$aRebets, Yuriy$$b0
000858501 245__ $$aCharacterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions
000858501 260__ $$aLausanne$$bFrontiers Media$$c2018
000858501 3367_ $$2DRIVER$$aarticle
000858501 3367_ $$2DataCite$$aOutput Types/Journal article
000858501 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1544794297_1951
000858501 3367_ $$2BibTeX$$aARTICLE
000858501 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000858501 3367_ $$00$$2EndNote$$aJournal Article
000858501 520__ $$aAlternative sigma factors control numerous aspects of bacterial life, including adaptation to physiological stresses, morphological development, persistence states and virulence. This is especially true for the physiologically complex actinobacteria. Here we report the development of a robust gene deletions system for Streptomyces lividans TK24 based on a BAC library combined with the λ-Red recombination technique. The developed system was validated by systematically deleting the most highly expressed genes encoding alternative sigma factors and several other regulatory genes within the chromosome of S. lividans TK24. To demonstrate the possibility of large scale genomic manipulations, the major part of the undecylprodigiosin gene cluster was deleted as well. The resulting mutant strains were characterized in terms of morphology, growth parameters, secondary metabolites production and response to thiol-oxidation and cell-wall stresses. Deletion of SLIV_12645 gene encoding S. coelicolor SigR1 ortholog has the most prominent phenotypic effect, resulted in overproduction of actinorhodin and coelichelin P1 and increased sensitivity to diamide. The secreted proteome analysis of SLIV_12645 mutant revealed SigR1 influence on trafficking of proteins involved in cell wall biogenesis and refactoring. The reported here gene deletion system will further facilitate work on S. lividans strain improvement as a host for either secondary metabolites or protein production and will contribute to basic research in streptomycetes physiology, morphological development, secondary metabolism. On the other hand, the systematic deletion of sigma factors encoding genes demonstrates the complexity and conservation of regulatory processes conducted by sigma factors in streptomycetes.
000858501 536__ $$0G:(DE-HGF)POF3-581$$a581 - Biotechnology (POF3-581)$$cPOF3-581$$fPOF III$$x0
000858501 588__ $$aDataset connected to CrossRef
000858501 7001_ $$0P:(DE-HGF)0$$aTsolis, Konstantinos C.$$b1
000858501 7001_ $$0P:(DE-HGF)0$$aGuðmundsdóttir, Elísabet Eik$$b2
000858501 7001_ $$0P:(DE-Juel1)162380$$aKoepff, Joachim$$b3
000858501 7001_ $$0P:(DE-HGF)0$$aWawiernia, Beata$$b4
000858501 7001_ $$0P:(DE-HGF)0$$aBusche, Tobias$$b5
000858501 7001_ $$0P:(DE-Juel1)168202$$aBleidt, Arne$$b6
000858501 7001_ $$0P:(DE-HGF)0$$aHorbal, Liliya$$b7
000858501 7001_ $$0P:(DE-HGF)0$$aMyronovskyi, Maksym$$b8
000858501 7001_ $$0P:(DE-HGF)0$$aAhmed, Yousra$$b9
000858501 7001_ $$0P:(DE-Juel1)129076$$aWiechert, Wolfgang$$b10$$ufzj
000858501 7001_ $$0P:(DE-Juel1)132913$$aRückert, Christian$$b11$$ufzj
000858501 7001_ $$0P:(DE-HGF)0$$aHamed, Mohamed B.$$b12
000858501 7001_ $$0P:(DE-HGF)0$$aBilyk, Bohdan$$b13
000858501 7001_ $$0P:(DE-HGF)0$$aAnné, Jozef$$b14
000858501 7001_ $$0P:(DE-HGF)0$$aFriðjónsson, Ólafur$$b15
000858501 7001_ $$0P:(DE-HGF)0$$aKalinowski, Jörn$$b16
000858501 7001_ $$0P:(DE-Juel1)129053$$aOldiges, Marco$$b17$$ufzj
000858501 7001_ $$0P:(DE-HGF)0$$aEconomou, Anastassios$$b18
000858501 7001_ $$0P:(DE-HGF)0$$aLuzhetskyy, Andriy$$b19$$eCorresponding author
000858501 773__ $$0PERI:(DE-600)2587354-4$$a10.3389/fmicb.2018.03033$$gVol. 9, p. 3033$$p3033$$tFrontiers in microbiology$$v9$$x1664-302X$$y2018
000858501 8564_ $$uhttps://juser.fz-juelich.de/record/858501/files/fmicb-09-03033-1.pdf$$yOpenAccess
000858501 8564_ $$uhttps://juser.fz-juelich.de/record/858501/files/fmicb-09-03033-1.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000858501 909CO $$ooai:juser.fz-juelich.de:858501$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000858501 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129076$$aForschungszentrum Jülich$$b10$$kFZJ
000858501 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)132913$$aForschungszentrum Jülich$$b11$$kFZJ
000858501 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129053$$aForschungszentrum Jülich$$b17$$kFZJ
000858501 9131_ $$0G:(DE-HGF)POF3-581$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vBiotechnology$$x0
000858501 9141_ $$y2018
000858501 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000858501 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000858501 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000858501 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFRONT MICROBIOL : 2017
000858501 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000858501 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000858501 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000858501 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000858501 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000858501 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000858501 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review
000858501 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000858501 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000858501 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000858501 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000858501 9201_ $$0I:(DE-Juel1)VDB55$$kIBT-1$$lBiotechnologie 1$$x0
000858501 980__ $$ajournal
000858501 980__ $$aVDB
000858501 980__ $$aUNRESTRICTED
000858501 980__ $$aI:(DE-Juel1)VDB55
000858501 9801_ $$aFullTexts