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@ARTICLE{Rebets:858501,
      author       = {Rebets, Yuriy and Tsolis, Konstantinos C. and
                      Guðmundsdóttir, Elísabet Eik and Koepff, Joachim and
                      Wawiernia, Beata and Busche, Tobias and Bleidt, Arne and
                      Horbal, Liliya and Myronovskyi, Maksym and Ahmed, Yousra and
                      Wiechert, Wolfgang and Rückert, Christian and Hamed,
                      Mohamed B. and Bilyk, Bohdan and Anné, Jozef and
                      Friðjónsson, Ólafur and Kalinowski, Jörn and Oldiges,
                      Marco and Economou, Anastassios and Luzhetskyy, Andriy},
      title        = {{C}haracterization of {S}igma {F}actor {G}enes in
                      {S}treptomyces lividans {TK}24 {U}sing a {G}enomic
                      {L}ibrary-{B}ased {A}pproach for {M}ultiple {G}ene
                      {D}eletions},
      journal      = {Frontiers in microbiology},
      volume       = {9},
      issn         = {1664-302X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2018-07372},
      pages        = {3033},
      year         = {2018},
      abstract     = {Alternative sigma factors control numerous aspects of
                      bacterial life, including adaptation to physiological
                      stresses, morphological development, persistence states and
                      virulence. This is especially true for the physiologically
                      complex actinobacteria. Here we report the development of a
                      robust gene deletions system for Streptomyces lividans TK24
                      based on a BAC library combined with the λ-Red
                      recombination technique. The developed system was validated
                      by systematically deleting the most highly expressed genes
                      encoding alternative sigma factors and several other
                      regulatory genes within the chromosome of S. lividans TK24.
                      To demonstrate the possibility of large scale genomic
                      manipulations, the major part of the undecylprodigiosin gene
                      cluster was deleted as well. The resulting mutant strains
                      were characterized in terms of morphology, growth
                      parameters, secondary metabolites production and response to
                      thiol-oxidation and cell-wall stresses. Deletion of
                      $SLIV_12645$ gene encoding S. coelicolor SigR1 ortholog has
                      the most prominent phenotypic effect, resulted in
                      overproduction of actinorhodin and coelichelin P1 and
                      increased sensitivity to diamide. The secreted proteome
                      analysis of $SLIV_12645$ mutant revealed SigR1 influence on
                      trafficking of proteins involved in cell wall biogenesis and
                      refactoring. The reported here gene deletion system will
                      further facilitate work on S. lividans strain improvement as
                      a host for either secondary metabolites or protein
                      production and will contribute to basic research in
                      streptomycetes physiology, morphological development,
                      secondary metabolism. On the other hand, the systematic
                      deletion of sigma factors encoding genes demonstrates the
                      complexity and conservation of regulatory processes
                      conducted by sigma factors in streptomycetes.},
      cin          = {IBT-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB55},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000452616600001},
      doi          = {10.3389/fmicb.2018.03033},
      url          = {https://juser.fz-juelich.de/record/858501},
}