TY  - JOUR
AU  - Aschenbrenner, Jennifer
AU  - Marx, Patrick
AU  - Pietruszka, Jörg
AU  - Marienhagen, Jan
TI  - Microbial production of natural and non‐natural monolignols with Escherichia coli
JO  - ChemBioChem
VL  - 20
IS  - 7
SN  - 1439-7633
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2018-07399
SP  - 949-954
PY  - 2019
N1  - Biotechnologie 1
AB  - Phenylpropanoids and phenylpropanoid‐derived plant polyphenols find numerous applications in the food and pharmaceutical industries. In recent years, several microbial platform organisms have been engineered towards producing such compounds. However, for the most part, microbial (poly)phenol production is inspired by nature, so naturally occurring compounds have predominantly been produced to date.Here we have taken advantage of the promiscuity of the enzymes involved in phenylpropanoid synthesis and exploited the versatility of an engineered Escherichia coli strain harboring a synthetic monolignol pathway to convert supplemented natural and unnatural phenylpropenoic acids into their corresponding monolignols. The performed biotransformations showed that this strain is able to catalyze the stepwise reduction of chemically interesting unnatural phenylpropenoic acids such as 3,4,5‐trimethoxycinnamic acid, 5‐bromoferulic acid, 2‐nitroferulic acid, and a “bicyclic” p‐coumaric acid derivative, in addition to six naturally occurring phenylpropenoic acids.
LB  - PUB:(DE-HGF)16
C6  - pmid:30537293
UR  - <Go to ISI:>//WOS:000462914700013
DO  - DOI:10.1002/cbic.201800673
UR  - https://juser.fz-juelich.de/record/858528
ER  -