000858580 001__ 858580
000858580 005__ 20240619091240.0
000858580 0247_ $$2Handle$$a2128/20963
000858580 0247_ $$2URN$$aurn:nbn:de:0001-2019030702
000858580 0247_ $$2ISSN$$a1866-1807
000858580 020__ $$a978-3-95806-369-3
000858580 037__ $$aFZJ-2018-07450
000858580 041__ $$aEnglish
000858580 1001_ $$0P:(DE-Juel1)164319$$aKreysing, Eva Maria$$b0$$eCorresponding author$$gfemale$$ufzj
000858580 245__ $$aCharacterization of the cell-substrate interface using surface plasmon resonance microscopy$$f- 2018-06-14
000858580 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2018
000858580 300__ $$aXIII, 260 S.
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000858580 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1545125583_2739
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000858580 4900_ $$aSchriften des Forschungszentrums Jülich. Reihe Schlüsseltechnologien / Key Technologies$$v189
000858580 502__ $$aRWTH Aachen, Diss., 2018$$bDr.$$cRWTH Aachen$$d2018
000858580 520__ $$aIn this thesis an improved surface plasmon resonance microscopy (SPRM) setup has been developed which combines a projector based SPRM widefield mode with several SPRM scanning modes for the investigation of the cell-substrate interface. Widefield SPRM can be used to image the cell-substrate adhesion areas qualitatively. Here, the resolution is strongly dependent on the light source. While coherent laser light gives rise to speckle noise, which frustrates the resolution of small cellular structures such as neuronal dendrites, using a projector as an incoherent light source allows for a high resolution imaging. Scanning SPRM can be used to determine the cell-substrate distance quantitatively. So far, the accuracy of these measurements was compromised by the assumption of a homogeneous refractive index (RI). In this thesis, it is shown that the RI can be extracted from the SPRM signal at each scanning point at the cell-substrate interface which allowed for an improvement of the distance accuracy by a factor of 25 compared to the standard analysis technique realizing a distance accuracy of up to 1.5 nm. The measurements of RI and distance were validated by several reference measurements. The RI of the cell gives interesting insights into the cellular structure and cellular processes. Scanning the cell-substrate interface, it could be shown that the RI profile of a cell can reveal the position of cell organelles and give quantitative values for their refractive indices while the scanning SPRM also allows for the reconstruction of the 3D structure of the basal cell membrane. [...]
000858580 536__ $$0G:(DE-HGF)POF3-552$$a552 - Engineering Cell Function (POF3-552)$$cPOF3-552$$fPOF III$$x0
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000858580 9141_ $$y2018
000858580 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)164319$$aForschungszentrum Jülich$$b0$$kFZJ
000858580 9131_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
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