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@ARTICLE{Petrovi:858914,
author = {Petrović, Dušan and Wang, Xue and Strodel, Birgit},
title = {{H}ow accurately do force fields represent protein side
chain ensembles?},
journal = {Proteins},
volume = {86},
number = {9},
issn = {0887-3585},
address = {New York, NY},
publisher = {Wiley-Liss},
reportid = {FZJ-2018-07750},
pages = {935 - 944},
year = {2018},
abstract = {Although the protein backbone is the most fundamental part
of the structure, the fine-tuning of side-chain
conformations is important for protein function, for
example, in protein-protein and protein-ligand interactions,
and also in enzyme catalysis. While several benchmarks
testing the performance of protein force fields for side
chain properties have already been published, they often
considered only a few force fields and were not tested
against the same experimental observables; hence, they are
not directly comparable. In this work, we explore the
ability of twelve force fields, which are different flavors
of AMBER, CHARMM, OPLS, or GROMOS, to reproduce average
rotamer angles and rotamer populations obtained from
extensive NMR studies of the 3 J and residual dipolar
coupling constants for two small proteins: ubiquitin and
GB3. Based on a total of 196 μs sampling time, our
results reveal that all force fields identify the correct
side chain angles, while the AMBER and CHARMM force fields
clearly outperform the OPLS and GROMOS force fields in
estimating rotamer populations. The three best force fields
for representing the protein side chain dynamics are AMBER
14SB, AMBER 99SB*-ILDN, and CHARMM36. Furthermore, we
observe that the side chain ensembles of buried amino acid
residues are generally more accurately represented than
those of the surface exposed residues.},
cin = {ICS-6 / JARA-HPC},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106 / $I:(DE-82)080012_20140620$},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551) /
Computational Enzyme Design $(jics69_20151101)$},
pid = {G:(DE-HGF)POF3-551 / $G:(DE-Juel1)jics69_20151101$},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29790608},
UT = {WOS:000446996700004},
doi = {10.1002/prot.25525},
url = {https://juser.fz-juelich.de/record/858914},
}