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@ARTICLE{Owen:858921,
author = {Owen, Michael C. and Kulig, Waldemar and Rog, Tomasz and
Vattulainen, Ilpo and Strodel, Birgit},
title = {{C}holesterol {P}rotects the {O}xidized {L}ipid {B}ilayer
from {W}ater {I}njury: {A}n {A}ll-{A}tom {M}olecular
{D}ynamics {S}tudy},
journal = {The journal of membrane biology},
volume = {251},
number = {3},
issn = {1432-1424},
address = {Heidelberg]},
publisher = {Springer},
reportid = {FZJ-2018-07757},
pages = {521 - 534},
year = {2018},
abstract = {In an effort to delineate how cholesterol protects membrane
structure under oxidative stress conditions, we monitored
the changes to the structure of lipid bilayers comprising 30
$mol\%$ cholesterol and an increasing concentration of Class
B oxidized 1-palmitoyl-2-oleoylphosphatidylcholine (POPC)
glycerophospholipids, namely,
1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine
(PoxnoPC), and
1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC),
using atomistic molecular dynamics simulations. Increasing
the content of oxidized phospholipids (oxPLs) from 0 to 60
$mol\%$ oxPL resulted in a characteristic reduction in
bilayer thickness and increase in area per lipid, thereby
increasing the exposure of the membrane hydrophobic region
to water. However, cholesterol was observed to help reduce
water injury by moving into the bilayer core and forming
more hydrogen bonds with the oxPLs. Cholesterol also resists
altering its tilt angle, helping to maintain membrane
integrity. Water that enters the 1-nm-thick core region
remains part of the bulk water on either side of the
bilayer, with relatively few water molecules able to
traverse through the bilayer. In cholesterol-rich membranes,
the bilayer does not form pores at concentrations of 60
$mol\%$ oxPL as was shown in previous simulations in the
absence of cholesterol.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29550877},
UT = {WOS:000437103200020},
doi = {10.1007/s00232-018-0028-9},
url = {https://juser.fz-juelich.de/record/858921},
}