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000859105 1001_ $$0P:(DE-HGF)0$$aBianchi, Federico$$b0
000859105 245__ $$aStructure of human telomere G-quadruplex in the presence of a model drug along the thermal unfolding pathway
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000859105 520__ $$aA multi-technique approach, combining circular dichroism spectroscopy, ultraviolet resonance Raman spectroscopy and small angle scattering techniques, has been deployed to elucidate how the structural features of the human telomeric G-quadruplex d[A(GGGTTA)3GGG] (Tel22) change upon thermal unfolding. The system is studied both in the free form and when it is bound to Actinomycin D (ActD), an anticancer ligand with remarkable conformational flexibility. We find that at room temperature binding of Tel22 with ActD involves end-stacking upon the terminal G-tetrad. Structural evidence for drug-driven dimerization of a significant fraction of the G-quadruplexes is provided. When the temperature is raised, both free and bound Tel22 undergo melting through a multi-state process. We show that in the intermediate states of Tel22 the conformational equilibrium is shifted toward the (3+1) hybrid-type, while a parallel structure is promoted in the complex. The unfolded state of the free Tel22 is consistent with a self-avoiding random-coil conformation, whereas the high-temperature state of the complex is observed to assume a quite compact form. Such an unprecedented high-temperature arrangement is caused by the persistent interaction between Tel22 and ActD, which stabilizes compact conformations even in the presence of large thermal structural fluctuations.
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000859105 7001_ $$00000-0001-5160-6844$$aComez, Lucia$$b1$$eCorresponding author
000859105 7001_ $$0P:(DE-Juel1)130542$$aBiehl, Ralf$$b2
000859105 7001_ $$0P:(DE-HGF)0$$aD’Amico, Francesco$$b3
000859105 7001_ $$0P:(DE-HGF)0$$aGessini, Alessandro$$b4
000859105 7001_ $$0P:(DE-Juel1)171805$$aLongo, Marialucia$$b5
000859105 7001_ $$0P:(DE-HGF)0$$aMasciovecchio, Claudio$$b6
000859105 7001_ $$0P:(DE-HGF)0$$aPetrillo, Caterina$$b7
000859105 7001_ $$0P:(DE-Juel1)130905$$aRadulescu, Aurel$$b8
000859105 7001_ $$0P:(DE-HGF)0$$aRossi, Barbara$$b9
000859105 7001_ $$0P:(DE-HGF)0$$aSacchetti, Francesco$$b10
000859105 7001_ $$0P:(DE-HGF)0$$aSebastiani, Federico$$b11
000859105 7001_ $$0P:(DE-Juel1)144823$$aViolini, Nicolo$$b12
000859105 7001_ $$0P:(DE-HGF)0$$aPaciaroni, Alessandro$$b13
000859105 773__ $$0PERI:(DE-600)1472175-2$$a10.1093/nar/gky1092$$gVol. 46, no. 22, p. 11927 - 11938$$n22$$p11927 - 11938$$tNucleic acids research$$v46$$x0301-5610$$y2018
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