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@ARTICLE{Bianchi:859105,
author = {Bianchi, Federico and Comez, Lucia and Biehl, Ralf and
D’Amico, Francesco and Gessini, Alessandro and Longo,
Marialucia and Masciovecchio, Claudio and Petrillo, Caterina
and Radulescu, Aurel and Rossi, Barbara and Sacchetti,
Francesco and Sebastiani, Federico and Violini, Nicolo and
Paciaroni, Alessandro},
title = {{S}tructure of human telomere {G}-quadruplex in the
presence of a model drug along the thermal unfolding
pathway},
journal = {Nucleic acids research},
volume = {46},
number = {22},
issn = {0301-5610},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {FZJ-2019-00049},
pages = {11927 - 11938},
year = {2018},
abstract = {A multi-technique approach, combining circular dichroism
spectroscopy, ultraviolet resonance Raman spectroscopy and
small angle scattering techniques, has been deployed to
elucidate how the structural features of the human telomeric
G-quadruplex d[A(GGGTTA)3GGG] (Tel22) change upon thermal
unfolding. The system is studied both in the free form and
when it is bound to Actinomycin D (ActD), an anticancer
ligand with remarkable conformational flexibility. We find
that at room temperature binding of Tel22 with ActD involves
end-stacking upon the terminal G-tetrad. Structural evidence
for drug-driven dimerization of a significant fraction of
the G-quadruplexes is provided. When the temperature is
raised, both free and bound Tel22 undergo melting through a
multi-state process. We show that in the intermediate states
of Tel22 the conformational equilibrium is shifted toward
the (3+1) hybrid-type, while a parallel structure is
promoted in the complex. The unfolded state of the free
Tel22 is consistent with a self-avoiding random-coil
conformation, whereas the high-temperature state of the
complex is observed to assume a quite compact form. Such an
unprecedented high-temperature arrangement is caused by the
persistent interaction between Tel22 and ActD, which
stabilizes compact conformations even in the presence of
large thermal structural fluctuations.},
cin = {JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {570},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF3-6G15 /
G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30407585},
UT = {WOS:000456714000026},
doi = {10.1093/nar/gky1092},
url = {https://juser.fz-juelich.de/record/859105},
}