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@ARTICLE{Bhm:859200,
      author       = {Böhm, Philip and Koutsioumpas, Alexandros and Moulin,
                      Jean-François and Rädler, Joachim O. and Sackmann, Erich
                      and Nickel, Bert},
      title        = {{P}robing the {I}nterface {S}tructure of {A}dhering {C}ells
                      by {C}ontrast {V}ariation {N}eutron {R}eflectometry},
      journal      = {Langmuir},
      volume       = {35},
      number       = {2},
      issn         = {1520-5827},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {FZJ-2019-00090},
      pages        = {513},
      year         = {2019},
      abstract     = {Cellular adhesion is a central element in tissue mechanics,
                      biological cell–cell signaling, and cell motility. In this
                      context, the cell–substrate distance has been investigated
                      in the past by studying natural cells and biomimetic cell
                      models adhering on solid substrates. The amount of water in
                      the membrane substrate gap, however, is difficult to
                      determine. Here, we present a neutron reflectivity (NR)
                      structural study of confluent epithelial cell monolayers on
                      silicon substrates. In order to ensure valid in vitro
                      conditions, we developed a cell culture sample chamber
                      allowing us to grow and cultivate cells under proper cell
                      culture conditions while performing in vitro neutron
                      reflectivity measurements. The cell chamber also enabled
                      perfusion with cell medium and hence allowed for contrast
                      variation in situ by sterile exchange of buffer with
                      different H2O-to-D2O ratio. Contrast variation reduces the
                      ambiguity of data modeling for determining the thickness and
                      degree of hydration of the interfacial cleft between the
                      adherent cells and the substrate. Our data suggest a
                      three-layer interfacial organization. The first layer bound
                      to the silicon surface interface is in agreement with a very
                      dense protein film with a thickness of 9 ± 2 nm, followed
                      by a highly hydrated 24 ± 4 nm thick layer, and a several
                      tens of nanometers thick layer attributed to the composite
                      membrane. Hence, the results provide clear evidence of a
                      highly hydrated intermediate region between the composite
                      cell membrane and the substrate, reminiscent of the basal
                      lamina.},
      cin          = {JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)JCNS-1-20110106},
      pnm          = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
                      Neutron Research (JCNS) (POF3-623)},
      pid          = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
      experiment   = {EXP:(DE-MLZ)MARIA-20140101 / EXP:(DE-MLZ)REFSANS-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30518215},
      UT           = {WOS:000456349700023},
      doi          = {10.1021/acs.langmuir.8b02228},
      url          = {https://juser.fz-juelich.de/record/859200},
}