Home > Publications database > Probing the Interface Structure of Adhering Cells by Contrast Variation Neutron Reflectometry > print

001     859200
005     20210130000209.0
024 7 _ |a 10.1021/acs.langmuir.8b02228
|2 doi
024 7 _ |a 0743-7463
|2 ISSN
024 7 _ |a 1520-5827
|2 ISSN
024 7 _ |a pmid:30518215
|2 pmid
024 7 _ |a WOS:000456349700023
|2 WOS
024 7 _ |a altmetric:52942532
|2 altmetric
037 _ _ |a FZJ-2019-00090
082 _ _ |a 540
100 1 _ |a Böhm, Philip
|0 P:(DE-HGF)0
|b 0
245 _ _ |a Probing the Interface Structure of Adhering Cells by Contrast Variation Neutron Reflectometry
260 _ _ |a Washington, DC
|c 2019
|b ACS Publ.
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1547715256_16920
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Cellular adhesion is a central element in tissue mechanics, biological cell–cell signaling, and cell motility. In this context, the cell–substrate distance has been investigated in the past by studying natural cells and biomimetic cell models adhering on solid substrates. The amount of water in the membrane substrate gap, however, is difficult to determine. Here, we present a neutron reflectivity (NR) structural study of confluent epithelial cell monolayers on silicon substrates. In order to ensure valid in vitro conditions, we developed a cell culture sample chamber allowing us to grow and cultivate cells under proper cell culture conditions while performing in vitro neutron reflectivity measurements. The cell chamber also enabled perfusion with cell medium and hence allowed for contrast variation in situ by sterile exchange of buffer with different H2O-to-D2O ratio. Contrast variation reduces the ambiguity of data modeling for determining the thickness and degree of hydration of the interfacial cleft between the adherent cells and the substrate. Our data suggest a three-layer interfacial organization. The first layer bound to the silicon surface interface is in agreement with a very dense protein film with a thickness of 9 ± 2 nm, followed by a highly hydrated 24 ± 4 nm thick layer, and a several tens of nanometers thick layer attributed to the composite membrane. Hence, the results provide clear evidence of a highly hydrated intermediate region between the composite cell membrane and the substrate, reminiscent of the basal lamina.
536 _ _ |0 G:(DE-HGF)POF3-6G15
|f POF III
|x 0
|c POF3-6G15
|a 6G15 - FRM II / MLZ (POF3-6G15)
536 _ _ |a 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
|0 G:(DE-HGF)POF3-6G4
|c POF3-623
|f POF III
|x 1
588 _ _ |a Dataset connected to CrossRef
650 2 7 |a Biology
|0 V:(DE-MLZ)SciArea-160
|2 V:(DE-HGF)
|x 0
650 1 7 |a Health and Life
|0 V:(DE-MLZ)GC-130-2016
|2 V:(DE-HGF)
|x 0
693 _ _ |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
|e MARIA: Magnetic reflectometer with high incident angle
|f NL5N
|1 EXP:(DE-MLZ)FRMII-20140101
|0 EXP:(DE-MLZ)MARIA-20140101
|5 EXP:(DE-MLZ)MARIA-20140101
|6 EXP:(DE-MLZ)NL5N-20140101
|x 0
693 _ _ |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
|e REFSANS: Horizontal TOF Reflectometer with GISANS option
|f NL2b
|1 EXP:(DE-MLZ)FRMII-20140101
|0 EXP:(DE-MLZ)REFSANS-20140101
|5 EXP:(DE-MLZ)REFSANS-20140101
|6 EXP:(DE-MLZ)NL2b-20140101
|x 1
700 1 _ |a Koutsioumpas, Alexandros
|0 P:(DE-Juel1)158075
|b 1
700 1 _ |a Moulin, Jean-François
|0 P:(DE-HGF)0
|b 2
700 1 _ |a Rädler, Joachim O.
|0 P:(DE-HGF)0
|b 3
700 1 _ |a Sackmann, Erich
|0 P:(DE-HGF)0
|b 4
700 1 _ |a Nickel, Bert
|0 0000-0002-0254-8841
|b 5
|e Corresponding author
773 _ _ |a 10.1021/acs.langmuir.8b02228
|g p. acs.langmuir.8b02228
|0 PERI:(DE-600)2005937-1
|n 2
|p 513
|t Langmuir
|v 35
|y 2019
|x 1520-5827
856 4 _ |u https://juser.fz-juelich.de/record/859200/files/acs.langmuir.8b02228.pdf
|y Restricted
856 4 _ |u https://juser.fz-juelich.de/record/859200/files/acs.langmuir.8b02228.pdf?subformat=pdfa
|x pdfa
|y Restricted
909 C O |o oai:juser.fz-juelich.de:859200
|p VDB:MLZ
|p VDB
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 1
|6 P:(DE-Juel1)158075
913 1 _ |a DE-HGF
|9 G:(DE-HGF)POF3-6G15
|x 0
|4 G:(DE-HGF)POF
|v FRM II / MLZ
|1 G:(DE-HGF)POF3-6G0
|0 G:(DE-HGF)POF3-6G15
|3 G:(DE-HGF)POF3
|2 G:(DE-HGF)POF3-600
|b Forschungsbereich Materie
|l Großgeräte: Materie
913 1 _ |a DE-HGF
|b Forschungsbereich Materie
|l Von Materie zu Materialien und Leben
|1 G:(DE-HGF)POF3-620
|0 G:(DE-HGF)POF3-623
|2 G:(DE-HGF)POF3-600
|v Facility topic: Neutrons for Research on Condensed Matter
|9 G:(DE-HGF)POF3-6G4
|x 1
|4 G:(DE-HGF)POF
|3 G:(DE-HGF)POF3
914 1 _ |y 2019
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0310
|2 StatID
|b NCBI Molecular Biology Database
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b LANGMUIR : 2017
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
915 _ _ |a WoS
|0 StatID:(DE-HGF)0110
|2 StatID
|b Science Citation Index
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
915 _ _ |a WoS
|0 StatID:(DE-HGF)0111
|2 StatID
|b Science Citation Index Expanded
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1150
|2 StatID
|b Current Contents - Physical, Chemical and Earth Sciences
915 _ _ |a IF < 5
|0 StatID:(DE-HGF)9900
|2 StatID
920 _ _ |l yes
920 1 _ |0 I:(DE-Juel1)JCNS-FRM-II-20110218
|k JCNS-FRM-II
|l JCNS-FRM-II
|x 0
920 1 _ |0 I:(DE-Juel1)JCNS-1-20110106
|k Neutronenstreuung ; JCNS-1
|l Neutronenstreuung
|x 1
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-Juel1)JCNS-FRM-II-20110218
980 _ _ |a I:(DE-Juel1)JCNS-1-20110106
980 _ _ |a UNRESTRICTED

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21