000859326 001__ 859326
000859326 005__ 20210130000233.0
000859326 0247_ $$2doi$$a10.1016/j.biomaterials.2018.10.020
000859326 0247_ $$2ISSN$$a0142-9612
000859326 0247_ $$2ISSN$$a1878-5905
000859326 0247_ $$2Handle$$a2128/21193
000859326 0247_ $$2pmid$$apmid:30502967
000859326 0247_ $$2WOS$$aWOS:000456902000050
000859326 0247_ $$2altmetric$$aaltmetric:54172913
000859326 037__ $$aFZJ-2019-00195
000859326 082__ $$a570
000859326 1001_ $$0P:(DE-HGF)0$$aSiewert, C.$$b0
000859326 245__ $$aInvestigation of charge ratio variation in mRNA – DEAE-dextran polyplex delivery systems
000859326 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2019
000859326 3367_ $$2DRIVER$$aarticle
000859326 3367_ $$2DataCite$$aOutput Types/Journal article
000859326 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1547562168_18954
000859326 3367_ $$2BibTeX$$aARTICLE
000859326 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000859326 3367_ $$00$$2EndNote$$aJournal Article
000859326 520__ $$amRNA pharmaceuticals represent a new class of therapeutics, with applications, in cancer vaccination, tumour therapy and protein substitution. Formulations are required to deliver messenger RNA (mRNA) to the target sites where induction of genetic transfection following receptor mediated cell uptake & translation is required.In the current study, the cationic polysaccharide diethylaminoethylen (DEAE) – Dextran was selected as a model system carrier for the investigation of polyplex nanoparticle formation together with mRNA as a function of the molar ratio of the components. The structure of the mRNA/Dextran colloids was investigated as a function of the polymer-to-mRNA ratio and correlated with the biological activity determined by cellular transfection with luciferase coding mRNA. Dynamic light scattering (DLS), small angle x-ray scattering (SAXS), and small angle neutron scattering (SANS) with deuterium contrast variation were used to achieve structural insight into the systems. Similarly to previously investigated lipid based systems, colloidally stable particles with confined size were obtained with either excess of positive or negative charge. Highest activity was obtained with positive charge excess. From the scattering experiments information on the internal organization inside the polymer/mRNA systems was derived. Indication for the presence of structural elements in the length scale of ten to 20 nm were found in the excess of dextran, which could be due to either excess or particulate polymer. Information on the molecular organization of the mRNA nanoparticle products may provide a valuable basis for defining critical quality attributes of drug products for pharmaceutical application.
000859326 536__ $$0G:(DE-HGF)POF3-6G4$$a6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)$$cPOF3-623$$fPOF III$$x0
000859326 536__ $$0G:(DE-HGF)POF3-6G15$$a6G15 - FRM II / MLZ (POF3-6G15)$$cPOF3-6G15$$fPOF III$$x1
000859326 588__ $$aDataset connected to CrossRef
000859326 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000859326 65027 $$0V:(DE-MLZ)SciArea-190$$2V:(DE-HGF)$$aMedicine$$x1
000859326 65017 $$0V:(DE-MLZ)GC-130-2016$$2V:(DE-HGF)$$aHealth and Life$$x0
000859326 693__ $$0EXP:(DE-MLZ)KWS2-20140101$$1EXP:(DE-MLZ)FRMII-20140101$$5EXP:(DE-MLZ)KWS2-20140101$$6EXP:(DE-MLZ)NL3ao-20140101$$aForschungs-Neutronenquelle Heinz Maier-Leibnitz $$eKWS-2: Small angle scattering diffractometer$$fNL3ao$$x0
000859326 7001_ $$0P:(DE-HGF)0$$aHaas, H.$$b1
000859326 7001_ $$0P:(DE-HGF)0$$aNawroth, T.$$b2
000859326 7001_ $$0P:(DE-HGF)0$$aZiller, A.$$b3
000859326 7001_ $$0P:(DE-HGF)0$$aNogueira, S. S.$$b4
000859326 7001_ $$0P:(DE-HGF)0$$aSchroer, M. A.$$b5
000859326 7001_ $$0P:(DE-HGF)0$$aBlanchet, C. E.$$b6
000859326 7001_ $$0P:(DE-HGF)0$$aSvergun, D. I.$$b7
000859326 7001_ $$0P:(DE-Juel1)130905$$aRadulescu, Aurel$$b8
000859326 7001_ $$0P:(DE-HGF)0$$aBates, F.$$b9
000859326 7001_ $$0P:(DE-HGF)0$$aHuesemann, Y.$$b10
000859326 7001_ $$0P:(DE-HGF)0$$aRadsak, M. P.$$b11
000859326 7001_ $$0P:(DE-HGF)0$$aSahin, U.$$b12
000859326 7001_ $$0P:(DE-HGF)0$$aLangguth, P.$$b13$$eCorresponding author
000859326 773__ $$0PERI:(DE-600)2004010-6$$a10.1016/j.biomaterials.2018.10.020$$gVol. 192, p. 612 - 620$$p612 - 620$$tBiomaterials$$v192$$x0142-9612$$y2019
000859326 8564_ $$uhttps://juser.fz-juelich.de/record/859326/files/1-s2.0-S0142961218307348-main.pdf$$yOpenAccess
000859326 8564_ $$uhttps://juser.fz-juelich.de/record/859326/files/1-s2.0-S0142961218307348-main.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000859326 909CO $$ooai:juser.fz-juelich.de:859326$$pdnbdelivery$$pVDB$$pVDB:MLZ$$pdriver$$popen_access$$popenaire
000859326 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)130905$$aForschungszentrum Jülich$$b8$$kFZJ
000859326 9131_ $$0G:(DE-HGF)POF3-623$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G4$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vFacility topic: Neutrons for Research on Condensed Matter$$x0
000859326 9131_ $$0G:(DE-HGF)POF3-6G15$$1G:(DE-HGF)POF3-6G0$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G15$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vFRM II / MLZ$$x1
000859326 9141_ $$y2019
000859326 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000859326 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000859326 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000859326 915__ $$0LIC:(DE-HGF)CCBYNCND4$$2HGFVOC$$aCreative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0
000859326 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOMATERIALS : 2017
000859326 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000859326 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000859326 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000859326 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000859326 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000859326 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bBIOMATERIALS : 2017
000859326 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000859326 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000859326 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000859326 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000859326 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000859326 920__ $$lyes
000859326 9201_ $$0I:(DE-Juel1)JCNS-FRM-II-20110218$$kJCNS-FRM-II$$lJCNS-FRM-II$$x0
000859326 9201_ $$0I:(DE-Juel1)JCNS-1-20110106$$kNeutronenstreuung ; JCNS-1$$lNeutronenstreuung $$x1
000859326 980__ $$ajournal
000859326 980__ $$aVDB
000859326 980__ $$aUNRESTRICTED
000859326 980__ $$aI:(DE-Juel1)JCNS-FRM-II-20110218
000859326 980__ $$aI:(DE-Juel1)JCNS-1-20110106
000859326 9801_ $$aFullTexts