000859958 001__ 859958
000859958 005__ 20240619092117.0
000859958 0247_ $$2doi$$a10.1021/acs.biomac.8b00196
000859958 0247_ $$2ISSN$$a1525-7797
000859958 0247_ $$2ISSN$$a1526-4602
000859958 0247_ $$2pmid$$apmid:29668268
000859958 0247_ $$2WOS$$aWOS:000438470800021
000859958 037__ $$aFZJ-2019-00764
000859958 082__ $$a570
000859958 1001_ $$0P:(DE-HGF)0$$aWeiss, Alessia C. G.$$b0
000859958 245__ $$aMicrofluidic Examination of the “Hard” Biomolecular Corona Formed on Engineered Particles in Different Biological Milieu
000859958 260__ $$aColumbus, Ohio$$bAmerican Chemical Soc.$$c2018
000859958 3367_ $$2DRIVER$$aarticle
000859958 3367_ $$2DataCite$$aOutput Types/Journal article
000859958 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1548426311_28091
000859958 3367_ $$2BibTeX$$aARTICLE
000859958 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000859958 3367_ $$00$$2EndNote$$aJournal Article
000859958 520__ $$aThe formation of a biomolecular corona around engineered particles determines, in large part, their biological behavior in vitro and in vivo. To gain a fundamental understanding of how particle design and the biological milieu influence the formation of the “hard” biomolecular corona, we conduct a series of in vitro studies using microfluidics. This setup allows the generation of a dynamic incubation environment with precise control over the applied flow rate, stream orientation, and channel dimensions, thus allowing accurate control of the fluid flow and the shear applied to the proteins and particles. We used mesoporous silica particles, poly(2-methacryloyloxyethylphosphorylcholine) (PMPC)-coated silica hybrid particles, and PMPC replica particles (obtained by removal of the silica particle templates), representing high-, intermediate-, and low-fouling particle systems, respectively. The protein source used in the experiments was either human serum or human full blood. The effects of flow, particle surface properties, incubation medium, and incubation time on the formation of the biomolecular corona formation are examined. Our data show that protein adhesion on particles is enhanced after incubation in human blood compared to human serum and that dynamic incubation leads to a more complex corona. By varying the incubation time from 2 s to 15 min, we demonstrate that the “hard” biomolecular corona is kinetically subdivided into two phases comprising a tightly bound layer of proteins interacting directly with the particle surface and a loosely associated protein layer. Understanding the influence of particle design parameters and biological factors on the corona composition, as well as its dynamic assembly, may facilitate more accurate prediction of corona formation and therefore assist in the design of advanced drug delivery vehicles.
000859958 536__ $$0G:(DE-HGF)POF3-551$$a551 - Functional Macromolecules and Complexes (POF3-551)$$cPOF3-551$$fPOF III$$x0
000859958 536__ $$0G:(DE-HGF)POF3-6G4$$a6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)$$cPOF3-623$$fPOF III$$x1
000859958 536__ $$0G:(DE-HGF)POF3-6215$$a6215 - Soft Matter, Health and Life Sciences (POF3-621)$$cPOF3-621$$fPOF III$$x2
000859958 588__ $$aDataset connected to CrossRef
000859958 7001_ $$00000-0002-0136-9403$$aKempe, Kristian$$b1
000859958 7001_ $$0P:(DE-Juel1)172658$$aFörster, Stephan$$b2
000859958 7001_ $$00000-0002-0197-497X$$aCaruso, Frank$$b3$$eCorresponding author
000859958 773__ $$0PERI:(DE-600)2006291-6$$a10.1021/acs.biomac.8b00196$$gVol. 19, no. 7, p. 2580 - 2594$$n7$$p2580 - 2594$$tBiomacromolecules$$v19$$x1526-4602$$y2018
000859958 8564_ $$uhttps://juser.fz-juelich.de/record/859958/files/acs.biomac.8b00196.pdf$$yRestricted
000859958 8564_ $$uhttps://juser.fz-juelich.de/record/859958/files/acs.biomac.8b00196.pdf?subformat=pdfa$$xpdfa$$yRestricted
000859958 909CO $$ooai:juser.fz-juelich.de:859958$$pVDB
000859958 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)172658$$aForschungszentrum Jülich$$b2$$kFZJ
000859958 9131_ $$0G:(DE-HGF)POF3-551$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vFunctional Macromolecules and Complexes$$x0
000859958 9131_ $$0G:(DE-HGF)POF3-623$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G4$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vFacility topic: Neutrons for Research on Condensed Matter$$x1
000859958 9131_ $$0G:(DE-HGF)POF3-621$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6215$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vIn-house research on the structure, dynamics and function of matter$$x2
000859958 9141_ $$y2018
000859958 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOMACROMOLECULES : 2017
000859958 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000859958 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000859958 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000859958 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000859958 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000859958 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000859958 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000859958 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000859958 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000859958 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000859958 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000859958 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000859958 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bBIOMACROMOLECULES : 2017
000859958 9201_ $$0I:(DE-Juel1)ICS-1-20110106$$kICS-1$$lNeutronenstreuung $$x0
000859958 9201_ $$0I:(DE-Juel1)JCNS-1-20110106$$kNeutronenstreuung ; JCNS-1$$lNeutronenstreuung $$x1
000859958 980__ $$ajournal
000859958 980__ $$aVDB
000859958 980__ $$aI:(DE-Juel1)ICS-1-20110106
000859958 980__ $$aI:(DE-Juel1)JCNS-1-20110106
000859958 980__ $$aUNRESTRICTED
000859958 981__ $$aI:(DE-Juel1)IBI-8-20200312
000859958 981__ $$aI:(DE-Juel1)JCNS-1-20110106