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@ARTICLE{Weiss:859958,
      author       = {Weiss, Alessia C. G. and Kempe, Kristian and Förster,
                      Stephan and Caruso, Frank},
      title        = {{M}icrofluidic {E}xamination of the “{H}ard”
                      {B}iomolecular {C}orona {F}ormed on {E}ngineered {P}articles
                      in {D}ifferent {B}iological {M}ilieu},
      journal      = {Biomacromolecules},
      volume       = {19},
      number       = {7},
      issn         = {1526-4602},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Soc.},
      reportid     = {FZJ-2019-00764},
      pages        = {2580 - 2594},
      year         = {2018},
      abstract     = {The formation of a biomolecular corona around engineered
                      particles determines, in large part, their biological
                      behavior in vitro and in vivo. To gain a fundamental
                      understanding of how particle design and the biological
                      milieu influence the formation of the “hard”
                      biomolecular corona, we conduct a series of in vitro studies
                      using microfluidics. This setup allows the generation of a
                      dynamic incubation environment with precise control over the
                      applied flow rate, stream orientation, and channel
                      dimensions, thus allowing accurate control of the fluid flow
                      and the shear applied to the proteins and particles. We used
                      mesoporous silica particles,
                      poly(2-methacryloyloxyethylphosphorylcholine) (PMPC)-coated
                      silica hybrid particles, and PMPC replica particles
                      (obtained by removal of the silica particle templates),
                      representing high-, intermediate-, and low-fouling particle
                      systems, respectively. The protein source used in the
                      experiments was either human serum or human full blood. The
                      effects of flow, particle surface properties, incubation
                      medium, and incubation time on the formation of the
                      biomolecular corona formation are examined. Our data show
                      that protein adhesion on particles is enhanced after
                      incubation in human blood compared to human serum and that
                      dynamic incubation leads to a more complex corona. By
                      varying the incubation time from 2 s to 15 min, we
                      demonstrate that the “hard” biomolecular corona is
                      kinetically subdivided into two phases comprising a tightly
                      bound layer of proteins interacting directly with the
                      particle surface and a loosely associated protein layer.
                      Understanding the influence of particle design parameters
                      and biological factors on the corona composition, as well as
                      its dynamic assembly, may facilitate more accurate
                      prediction of corona formation and therefore assist in the
                      design of advanced drug delivery vehicles.},
      cin          = {ICS-1 / Neutronenstreuung ; JCNS-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-1-20110106 / I:(DE-Juel1)JCNS-1-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551) /
                      6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
                      / 6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-551 / G:(DE-HGF)POF3-6G4 /
                      G:(DE-HGF)POF3-6215},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29668268},
      UT           = {WOS:000438470800021},
      doi          = {10.1021/acs.biomac.8b00196},
      url          = {https://juser.fz-juelich.de/record/859958},
}