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@ARTICLE{Simons:860092,
      author       = {Simons, Indra M. and Mohrlüder, Jeannine and Feederle,
                      Regina and Kremmer, Elisabeth and Zobel, Thomas and Dobner,
                      Jochen and Bleffert, Nicole and Hoffmann, Silke and
                      Willbold, Dieter},
      title        = {{T}he highly {GABARAP} specific rat monoclonal antibody
                      8{H}5 visualizes {GABARAP} in immunofluorescence imaging at
                      endogenous levels},
      journal      = {Scientific reports},
      volume       = {9},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {FZJ-2019-00878},
      pages        = {526},
      year         = {2019},
      abstract     = {The determination of unique functions of GABARAP
                      (gamma-aminobutyric acid type A receptor-associated
                      protein), a member of the highly conserved protein family of
                      mammalian autophagy-related 8 protein (mATG8), within
                      diverse cellular processes remains challenging. Because
                      available anti-GABARAP antibodies perform inadequate,
                      especially within various microscopy-based applications, we
                      aimed to develop an antibody that targets GABARAP but not
                      its close orthologs. Following the latest recommendations
                      for antibody validation including fluorescence protein
                      tagging, genetic and orthogonal strategies, we characterized
                      the resulting anti-GABARAP (8H5) antibody during confocal
                      immunofluorescence imaging in-depth. We compared the
                      antibody staining pattern with that obtained for
                      fluorescence protein tagged GABARAP, GABARAPL1 or GABARAPL2
                      each ectopically expressed in GABARAP knockout cells.
                      Furthermore, we imaged cells expressing all mATG8 family
                      members at endogenous levels and checked GABARAP knockout
                      cells for unspecific staining under fed or
                      macroautophagy-inducing conditions. Finally, we
                      simultaneously stained cells for endogenous GABARAP and the
                      common autophagosomal marker LC3B. Summarized, the presented
                      antibody shows high specificity for GABARAP without
                      cross-reactivity to other mATG8 family members in
                      immunofluorescence imaging making it a valuable tool for the
                      identification of unique GABARAP functions.},
      cin          = {ICS-6},
      ddc          = {600},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30679523},
      UT           = {WOS:000456553400091},
      doi          = {10.1038/s41598-018-36717-1},
      url          = {https://juser.fz-juelich.de/record/860092},
}