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@ARTICLE{Kreysing:860483,
      author       = {Kreysing, Eva and Hassani, Hossein and Hampe, Nico and
                      Offenhäusser, Andreas},
      title        = {{N}anometer-{R}esolved {M}apping of {C}ell–{S}ubstrate
                      {D}istances of {C}ontracting {C}ardiomyocytes {U}sing
                      {S}urface {P}lasmon {R}esonance {M}icroscopy},
      journal      = {ACS nano},
      volume       = {12},
      number       = {9},
      issn         = {1936-086X},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2019-01237},
      pages        = {8934 - 8942},
      year         = {2018},
      abstract     = {It has been shown that quantitative measurements of the
                      cell–substrate distance of steady cells are possible with
                      scanning surface plasmon resonance microscopy setups in
                      combination with an angle resolved analysis. However, the
                      accuracy of the determined cell–substrate distances as
                      well as the capabilities for the investigation of cell
                      dynamics remained limited due to the assumption of a
                      homogeneous refractive index of the cytosol. Strong spatial
                      or temporal deviations between the local refractive index
                      and the average value can result in errors in the calculated
                      cell–substrate distance of around 100 nm, while the
                      average accuracy was determined to 37 nm. Here, we present a
                      combination of acquisition and analysis techniques that
                      enables the measurement of the cell–substrate distance of
                      contractile cells as well as the study of intracellular
                      processes through changes in the refractive index at the
                      diffraction limit. By decoupling the measurement of the
                      cell–substrate distance and the refractive index of the
                      cytoplasm, we could increase the accuracy of the distance
                      measurement on average by a factor of 25 reaching 1.5 nm
                      under ideal conditions. We show a temporal and spatial
                      mapping of changes in the refractive index and the
                      cell–substrate distance which strongly correlate with the
                      action potentials and reconstruct the three-dimensional
                      profile of the basal cell membrane and its dynamics, while
                      we reached an actual measurement accuracy of 2.3 nm.},
      cin          = {ICS-7 / ICS-8},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-7-20110106 / I:(DE-Juel1)ICS-8-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30180539},
      UT           = {WOS:000445972400012},
      doi          = {10.1021/acsnano.8b01396},
      url          = {https://juser.fz-juelich.de/record/860483},
}