000860659 001__ 860659
000860659 005__ 20210130000552.0
000860659 037__ $$aFZJ-2019-01327
000860659 1001_ $$0P:(DE-Juel1)138266$$aSchrader, Tobias Erich$$b0$$eCorresponding author$$ufzj
000860659 1112_ $$aMondern Concepts Seminar, Universität Wien$$cWien$$d2018-06-21 - 2018-06-22$$wAustria
000860659 245__ $$aNeutron protein crystallography: New developments and recent application examples$$f2018-06-21 - 
000860659 260__ $$c2018
000860659 3367_ $$033$$2EndNote$$aConference Paper
000860659 3367_ $$2DataCite$$aOther
000860659 3367_ $$2BibTeX$$aINPROCEEDINGS
000860659 3367_ $$2ORCID$$aLECTURE_SPEECH
000860659 3367_ $$0PUB:(DE-HGF)31$$2PUB:(DE-HGF)$$aTalk (non-conference)$$btalk$$mtalk$$s1550063335_17466$$xInvited
000860659 3367_ $$2DINI$$aOther
000860659 502__ $$cUniversität Wien
000860659 520__ $$aWith the advent of new instruments (e. g. Imagine at HFIR, MANDI at SNS and BIODIFF at FRMII) neutron protein crystallography has seen a resurrection from the past pioneering work by Goldstein et al. A growing user community has greatly enhanced the outcome of the existing neutron diffractometers specialized on protein crystallography. Measurements at 100 K are now routinely possible at many neutron diffractometers. Efforts to increase the flux at the sample position and to reduce the background at the detector push the limit of crystal volumes down to 0.1 mm3. The neutron single crystal diffractometer BIODIFF at the research reactor Heinz Maier-Leibnitz (FRM II) is is a joint project of the Jülich Centre for Neutron Science (JCNS) and the FRM II. BIODIFF is designed as a monochromatic instrument with a narrow wavelength spread of less than 3 % where the central wavelength can be adapted between 2.7 and 5.6 Å. Some typical application examples to illustrate the power of neutron protein crystallography will be given. Time permitting I will report on two of my own research projects. In one collaboration with the TUM engineering department we try to optimize the crystallization of the protein Alcoholdehydrogenase (ADH) by mutations on the solvent exposed surface of the protein. The thermodynamic effects on the crystal stability are simulated with all-atom MD-simulations. The MD-results will be compared to the actually observed crystallization behaviour of these mutants. In a second project a crystallization apparatus is developed which starts where the crystallization for x-ray sized crystals ends: At a tiny crystal and known crystallization conditions for it. The apparatus is intended to be used to increase the size of this crystal by using it as a seed crystal being put into a mother liquor containing new supply of highly concentrated protein. Fine temperature control and an optical control of the actual crystal size will be provided with this apparatus. The idea behind this apparatus is of course the growth of crystals which have a sufficient volume for neutron protein crystallography.
000860659 536__ $$0G:(DE-HGF)POF3-6G15$$a6G15 - FRM II / MLZ (POF3-6G15)$$cPOF3-6G15$$fPOF III$$x0
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000860659 536__ $$0G:(DE-HGF)POF3-6215$$a6215 - Soft Matter, Health and Life Sciences (POF3-621)$$cPOF3-621$$fPOF III$$x2
000860659 65017 $$0V:(DE-MLZ)GC-130-2016$$2V:(DE-HGF)$$aHealth and Life$$x0
000860659 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000860659 693__ $$0EXP:(DE-MLZ)BIODIFF-20140101$$1EXP:(DE-MLZ)FRMII-20140101$$5EXP:(DE-MLZ)BIODIFF-20140101$$6EXP:(DE-MLZ)NL1-20140101$$aForschungs-Neutronenquelle Heinz Maier-Leibnitz $$eBIODIFF: Diffractometer for large unit cells$$fNL1$$x0
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000860659 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)138266$$aForschungszentrum Jülich$$b0$$kFZJ
000860659 9131_ $$0G:(DE-HGF)POF3-6G15$$1G:(DE-HGF)POF3-6G0$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G15$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vFRM II / MLZ$$x0
000860659 9131_ $$0G:(DE-HGF)POF3-623$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G4$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vFacility topic: Neutrons for Research on Condensed Matter$$x1
000860659 9131_ $$0G:(DE-HGF)POF3-621$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6215$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vIn-house research on the structure, dynamics and function of matter$$x2
000860659 9141_ $$y2019
000860659 920__ $$lyes
000860659 9201_ $$0I:(DE-Juel1)JCNS-FRM-II-20110218$$kJCNS-FRM-II$$lJCNS-FRM-II$$x0
000860659 9201_ $$0I:(DE-Juel1)JCNS-1-20110106$$kNeutronenstreuung ; JCNS-1$$lNeutronenstreuung $$x1
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000860659 980__ $$aI:(DE-Juel1)JCNS-1-20110106
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