TY  - JOUR
AU  - Katranidis, Alexandros
AU  - Fitter, Jörg
TI  - Single-Molecule Techniques and Cell-Free Protein Synthesis: A Perfect Marriage
JO  - Analytical chemistry
VL  - 91
IS  - 4
SN  - 1520-6882
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - FZJ-2019-01524
SP  - 2570 - 2576
PY  - 2019
AB  - Single-molecule techniques are nowadays an essential tool to study conformational changes as well as synthesis and folding of proteins. However, preparation of suitable protein samples is often time consuming and demanding. The rapid development of cell-free protein synthesis over the last years opened new perspectives for fast and easy sample preparation but this was not fully exploited until now. Here, we take a look at the advancements in sample preparation as well as in the development of technical approaches and analytical tools, which unavoidably lead to the combination of single-molecule techniques and cell-free protein synthesis. It’s an ideal combination that can unlock the full potential of studying complex biological processes in the near future.
LB  - PUB:(DE-HGF)16
C6  - pmid:30648382
UR  - <Go to ISI:>//WOS:000459642400002
DO  - DOI:10.1021/acs.analchem.8b03855
UR  - https://juser.fz-juelich.de/record/860872
ER  -