TY  - JOUR
AU  - Geinguenaud, Frédéric
AU  - Calandrini, Vania
AU  - Teixeira, José
AU  - Mayer, Claudine
AU  - Liquier, Jean
AU  - Lavelle, Christophe
AU  - Arluison, Véronique
TI  - Conformational transition of DNA bound to Hfq probed by infrared spectroscopy
JO  - Physical chemistry, chemical physics
VL  - 13
IS  - 3
SN  - 1463-9084
CY  - Cambridge
PB  - RSC Publ.66479
M1  - FZJ-2019-01834
SP  - 1222 - 1229
PY  - 2011
AB  - Hfq is a bacterial protein involved in RNA metabolism. Besides this, Hfq's role in DNA restructuring has also been suggested. Since this mechanism remains unclear, we examined the DNA conformation upon Hfq binding by combining vibrational spectroscopy and neutron scattering. Our analysis reveals that Hfq, which preferentially interacts with deoxyadenosine rich sequences, induces partial opening of dA–dT sequences accompanied by sugar repuckering of the dA strand and hence results in a heteronomous A/B duplex. Sugar repuckering is probably correlated with a global dehydration of the complex. By taking into account Hfq's preferential binding to A-tracts, which are commonly found in promoters, potential biological implications of Hfq binding to DNA are discussed.
LB  - PUB:(DE-HGF)16
C6  - pmid:21082116
UR  - <Go to ISI:>//WOS:000285750100048
DO  - DOI:10.1039/C0CP01084G
UR  - https://juser.fz-juelich.de/record/861352
ER  -