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@ARTICLE{Springer:861443,
      author       = {Springer, Ronald and Zielinski, Alexander and Pleschka,
                      Catharina and Hoffmann, Bernd and Merkel, Rudolf},
      title        = {{U}nbiased pattern analysis reveals highly diverse
                      responses of cytoskeletal systems to cyclic straining},
      journal      = {PLOS ONE},
      volume       = {14},
      number       = {3},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {FZJ-2019-01915},
      pages        = {e0210570 -},
      year         = {2019},
      abstract     = {In mammalian cells, actin, microtubules, and various types
                      of cytoplasmic intermediate filaments respond to external
                      stretching. Here, we investigated the underlying processes
                      in endothelial cells plated on soft substrates from silicone
                      elastomer. After cyclic stretch (0.13 Hz, $14\%$ strain
                      amplitude) for periods ranging from 5 min to 8 h, cells were
                      fixed and double-stained for microtubules and either actin
                      or vimentin. Cell images were analyzed by a two-step
                      routine. In the first step, micrographs were segmented for
                      potential fibrous structures. In the second step, the
                      resulting binary masks were auto- or cross-correlated.
                      Autocorrelation of segmented images provided a sensitive and
                      objective measure of orientational and translational order
                      of the different cytoskeletal systems. Aligning of
                      correlograms from individual cells removed the influence of
                      only partial alignment between cells and enabled
                      determination of intrinsic cytoskeletal order. We found that
                      cyclic stretching affected the actin cytoskeleton most,
                      microtubules less, and vimentin mostly only via
                      reorientation of the whole cell. Pharmacological disruption
                      of microtubules had barely any influence on actin ordering.
                      The similarity, i.e., cross-correlation, between vimentin
                      and microtubules was much higher than the one between actin
                      and microtubules. Moreover, prolonged cyclic stretching
                      slightly decoupled the cytoskeletal systems as it reduced
                      the cross-correlations in both cases. Finally, actin and
                      microtubules were more correlated at peripheral regions of
                      cells whereas vimentin and microtubules correlated more in
                      central regions.},
      cin          = {ICS-7},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30865622},
      UT           = {WOS:000461048900007},
      doi          = {10.1371/journal.pone.0210570},
      url          = {https://juser.fz-juelich.de/record/861443},
}