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@ARTICLE{Zhang:862092,
author = {Zhang, Yuting and Figueroa-Miranda, Gabriela and Lyu,
Zhaozi and Zafiu, Christian and Willbold, Dieter and
Offenhäusser, Andreas and Mayer, Dirk},
title = {{M}onitoring amyloid-β proteins aggregation based on
label-free aptasensor},
journal = {Sensors and actuators / B Chemical : an international
journal devoted to research and development of chemical
transducers an international journal devoted to research and
development of chemical transducers},
volume = {288},
issn = {0925-4005},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2019-02448},
pages = {535 - 542},
year = {2019},
abstract = {Amyloid-β oligomers (AβO) are important diagnostic
markers for Alzheimer's disease (AD) and potential
therapeutic targets for treating it. Herein, a simple and
label-free electrochemical biosensor is presented for the
specific recognition of AβO based on the binding of these
biomarkers to ssDNA aptamer receptors. The ssDNA aptamer
self-assembles to gold rod electrodes through Au-S
interactions and binds specifically to AβO. The novel
aptasensor shows a wide linear concentration range from 0.1
to 500 nM with a low detection limit of 0.03 nM as
derived from electrochemical impedance spectroscopy
invesastigations. Furthermore, owing to the high selectivity
among Aβ species, this label-free sensor was used to
monitor the process of Aβ protein aggregation, which was
validated by atomic force microscope analysis. In addition,
the aptasensor can be used to detect AβO in artificial CSF
with satisfying accuracy. To our knowledge, it is first
label-free aptasensor for an AβO assay based on EIS that
works in artificial CSF and can be used for monitoring Aβ
protein aggregation. These features, together with its easy
fabrication, operation convenience, and effective
regeneration, make our new aptasensor a promising tool for
the early detection of AD and drug development by monitoring
Aβ plaques degradation.},
cin = {ICS-8},
ddc = {620},
cid = {I:(DE-Juel1)ICS-8-20110106},
pnm = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
pid = {G:(DE-HGF)POF3-523},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000462468000067},
doi = {10.1016/j.snb.2019.03.049},
url = {https://juser.fz-juelich.de/record/862092},
}