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@ARTICLE{Sasse:862128,
author = {Sasse, Joelle and Kant, Josefine and Cole, Benjamin J. and
Klein, Andrew P. and Arsova, Borjana and Schlaepfer, Pascal
and Gao, Jian and Lewald, Kyle and Zhalnina, Kateryna and
Kosina, Suzanne and Bowen, Benjamin P. and Treen, Daniel and
Vogel, John and Visel, Axel and Watt, Michelle and Dangl,
Jeffery L. and Northen, Trent R.},
title = {{M}ultilab {E}co{FAB} study shows highly reproducible
physiology and depletion of soil metabolites by a model
grass},
journal = {The new phytologist},
volume = {222},
number = {2},
issn = {1469-8137},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2019-02484},
pages = {1149 - 1160},
year = {2019},
abstract = {There is a dynamic reciprocity between plants and their
environment: soil physiochemical properties influence plant
morphology and metabolism, and root morphology and exudates
shape the environment surrounding roots. Here, we
investigate the reproducibility of plant trait changes in
response to three growth environments. We utilized
fabricated ecosystem (EcoFAB) devices to grow the model
grass Brachypodium distachyon in three distinct media across
four laboratories: phosphate‐sufficient and ‐deficient
mineral media allowed assessment of the effects of phosphate
starvation, and a complex, sterile soil extract represented
a more natural environment with yet uncharacterized effects
on plant growth and metabolism. Tissue weight and phosphate
content, total root length, and root tissue and exudate
metabolic profiles were consistent across laboratories and
distinct between experimental treatments. Plants grown in
soil extract were morphologically and metabolically
distinct, with root hairs four times longer than with other
growth conditions. Further, plants depleted half of the
metabolites investigated from the soil extract. To interact
with their environment, plants not only adapt morphology and
release complex metabolite mixtures, but also selectively
deplete a range of soil‐derived metabolites. The EcoFABs
utilized here generated high interlaboratory
reproducibility, demonstrating their value in standardized
investigations of plant traits.},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30585637},
UT = {WOS:000465446300044},
doi = {10.1111/nph.15662},
url = {https://juser.fz-juelich.de/record/862128},
}