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@ARTICLE{Weiel:862389,
author = {Weiel, Marie and Reinartz, Ines and Schug, Alexander},
title = {{R}apid interpretation of small-angle {X}-ray scattering
data},
journal = {PLoS Computational Biology},
volume = {15},
number = {3},
issn = {1553-7358},
address = {San Francisco, Calif.},
publisher = {Public Library of Science},
reportid = {FZJ-2019-02717},
pages = {e1006900 -},
year = {2019},
abstract = {The fundamental aim of structural analyses in biophysics is
to reveal a mutual relation between a molecule’s dynamic
structure and its physiological function. Small-angle X-ray
scattering (SAXS) is an experimental technique for
structural characterization of macromolecules in solution
and enables time-resolved analysis of conformational changes
under physiological conditions. As such experiments measure
spatially averaged low-resolution scattering intensities
only, the sparse information obtained is not sufficient to
uniquely reconstruct a three-dimensional atomistic model.
Here, we integrate the information from SAXS into molecular
dynamics simulations using computationally efficient native
structure-based models. Dynamically fitting an initial
structure towards a scattering intensity, such simulations
produce atomistic models in agreement with the target data.
In this way, SAXS data can be rapidly interpreted while
retaining physico-chemical knowledge and sampling power of
the underlying force field. We demonstrate our method’s
performance using the example of three protein systems.
Simulations are faster than full molecular dynamics
approaches by more than two orders of magnitude and
consistently achieve comparable accuracy. Computational
demands are reduced sufficiently to run the simulations on
commodity desktop computers instead of high-performance
computing systems. These results underline that
scattering-guided structure-based simulations provide a
suitable framework for rapid early-stage refinement of
structures towards SAXS data with particular focus on
minimal computational resources and time.},
cin = {JSC / NIC},
ddc = {610},
cid = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406},
pnm = {511 - Computational Science and Mathematical Methods
(POF3-511)},
pid = {G:(DE-HGF)POF3-511},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30901335},
UT = {WOS:000463877900064},
doi = {10.1371/journal.pcbi.1006900},
url = {https://juser.fz-juelich.de/record/862389},
}