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@ARTICLE{Riest:862616,
author = {Riest, Jonas and Naegele, Gerhard and Liu, Yun and Wagner,
Norman J. and Godfrin, P. Douglas},
title = {{S}hort-time dynamics of lysozyme solutions with competing
short-range attraction and long-range repulsion:
{E}xperiment and theory},
journal = {The journal of chemical physics},
volume = {148},
number = {6},
issn = {1089-7690},
address = {Melville, NY},
publisher = {American Institute of Physics},
reportid = {FZJ-2019-02885},
pages = {065101 -},
year = {2018},
abstract = {Recently, atypical static features of microstructural
ordering in low-salinity lysozyme protein solutions have
been extensively explored experimentally and explained
theoretically based on a short-range attractive plus
long-range repulsive (SALR) interaction potential. However,
the protein dynamics and the relationship to the atypical
SALR structure remain to be demonstrated. Here, the
applicability of semi-analytic theoretical methods
predicting diffusion properties and viscosity in isotropic
particle suspensions to low-salinity lysozyme protein
solutions is tested. Using the interaction potential
parameters previously obtained from static structure factor
measurements, our results of Monte Carlo simulations
representing seven experimental lysoyzme samples indicate
that they exist either in dispersed fluid or random
percolated states. The self-consistent Zerah-Hansen scheme
is used to describe the static structure factor, S(q), which
is the input to our calculation schemes for the short-time
hydrodynamic function, H(q), and the zero-frequency
viscosity η. The schemes account for hydrodynamic
interactions included on an approximate level. Theoretical
predictions for H(q) as a function of the wavenumber q
quantitatively agree with experimental results at small
protein concentrations obtained using neutron spin echo
measurements. At higher concentrations, qualitative
agreement is preserved although the calculated hydrodynamic
functions are overestimated. We attribute the differences
for higher concentrations and lower temperatures to
translational-rotational diffusion coupling induced by the
shape and interaction anisotropy of particles and clusters,
patchiness of the lysozyme particle surfaces, and the
intra-cluster dynamics, features not included in our simple
globular particle model. The theoretical results for the
solution viscosity, η, are in qualitative agreement with
our experimental data even at higher concentrations. We
demonstrate that semi-quantitative predictions of diffusion
properties and viscosity of solutions of globular proteins
are possible given only the equilibrium structure factor of
proteins. Furthermore, we explore the effects of changing
the attraction strength on H(q) and η},
cin = {ICS-3},
ddc = {530},
cid = {I:(DE-Juel1)ICS-3-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29448794},
UT = {WOS:000425299800035},
doi = {10.1063/1.5016517},
url = {https://juser.fz-juelich.de/record/862616},
}