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@ARTICLE{Westerwalbesloh:862635,
      author       = {Westerwalbesloh, Christoph and Brehl, Carl and Weber,
                      Sophie and Probst, Christopher and Widzgowski, Janka and
                      Grünberger, Alexander and Pfaff, Christian and Nedbal,
                      Ladislav and Kohlheyer, Dietrich},
      title        = {{A} microfluidic photobioreactor for simultaneous
                      observation and cultivation of single microalgal cells or
                      cell aggregates},
      journal      = {PLOS ONE},
      volume       = {14},
      number       = {4},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {FZJ-2019-02897},
      pages        = {e0216093 -},
      year         = {2019},
      abstract     = {Microalgae are an ubiquitous and powerful driver of
                      geochemical cycles which have formed Earth’s biosphere
                      since early in the evolution. Lately, microalgal research
                      has been strongly stimulated by economic potential expected
                      in biofuels, wastewater treatment, and high-value products.
                      Similar to bacteria and other microorganisms, most work so
                      far has been performed on the level of suspensions which
                      typically contain millions of algal cells per millilitre.
                      The thus obtained macroscopic parameters average cells,
                      which may be in various phases of their cell cycle or even,
                      in the case of microbial consortia, cells of different
                      species. This averaging may obscure essential features which
                      may be needed for the correct understanding and
                      interpretation of investigated processes. In contrast to
                      these conventional macroscopic cultivation and measuring
                      tools, microfluidic single-cell cultivation systems
                      represent an excellent alternative to study individual cells
                      or a small number of mutually interacting cells in a
                      well-defined environment. A novel microfluidic
                      photobioreactor was developed and successfully tested by the
                      photoautotrophic cultivation of Chlorella sorokiniana. The
                      reported microbioreactor facilitates automated long-term
                      cultivation of algae with controlled temperature and with an
                      illumination adjustable over a wide range of photon flux
                      densities. Chemical composition of the medium in the
                      microbioreactor can be stabilised or modulated rapidly to
                      study the response of individual cells. Furthermore, the
                      algae are cultivated in one focal plane and separate
                      chambers, enabling single-cell level investigation of over
                      100 microcolonies in parallel. The developed platform can be
                      used for systematic growth studies, medium screening,
                      species interaction studies, and the thorough investigation
                      of light-dependent growth kinetics},
      cin          = {IBG-1 / IBG-2},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IBG-2-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31034529},
      UT           = {WOS:000466131200034},
      doi          = {10.1371/journal.pone.0216093},
      url          = {https://juser.fz-juelich.de/record/862635},
}