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@ARTICLE{Koch:862824,
      author       = {Koch, Maximilian and Schiedung, Henning and Siebers, Nina
                      and McGovern, Sean and Hofmann, Diana and Vereecken, Harry
                      and Amelung, Wulf},
      title        = {{Q}uantitative imaging of 33{P} in plant materials using
                      14{C} polymerreferences},
      journal      = {Analytical and bioanalytical chemistry},
      volume       = {411},
      number       = {6},
      issn         = {0016-1152},
      address      = {Heidelberg},
      publisher    = {Springer},
      reportid     = {FZJ-2019-03025},
      pages        = {1253-1260},
      year         = {2019},
      abstract     = {Phosphorus (P) research still lacks techniques for rapid
                      imaging of P use and allocation in different soil, sediment,
                      and biologicalsystems in a quantitative manner. In this
                      study, we describe a time-saving and cost-efficient digital
                      autoradiographic method for insitu quantitative imaging of
                      33P radioisotopes in plant materials. Our method combines
                      autoradiography of the radiotracerapplications with
                      additions of commercially available 14C polymer references
                      to obtain 33P activities in a quantitative mannerup to 2000
                      Bq cm−2. Our data show that linear standard regressions
                      for both radioisotopes are obtained, allowing the
                      establishmentof photostimulated luminescence equivalence
                      between both radioisotopes with a factor of 9.73. Validating
                      experimentsrevealed a good agreement between the calculated
                      and applied 33P activity (R2 = 0.96). This finding was also
                      valid for the coexposureof 14C polymer references and 33P
                      radioisotope specific activities in excised plant leaves for
                      both maize (R2 = 0.99) andwheat (R2 = 0.99). The outlined
                      autoradiographic quantification procedure retrieved $100\%$
                      ± $12\%$ of the 33P activity in the plantleaves,
                      irrespective of plant tissue density. The simplicity of this
                      methodology opens up new perspectives for fast
                      quantitativeimaging of 33P in biological systems and likely,
                      thus, also for other environmental compartments.},
      cin          = {IBG-3},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {255 - Terrestrial Systems: From Observation to Prediction
                      (POF3-255)},
      pid          = {G:(DE-HGF)POF3-255},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30617405},
      UT           = {WOS:000458358400013},
      doi          = {10.1007/s00216-018-1557-x},
      url          = {https://juser.fz-juelich.de/record/862824},
}