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000862852 1001_ $$0P:(DE-HGF)0$$aJunker, Niklas O.$$b0
000862852 245__ $$aImpact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules
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000862852 520__ $$aEffects of molecular crowding on structural and dynamical properties of biological macromolecules do depend on the concentration of crowding agents but also on the molecular mass and the structural compactness of the crowder molecules. By employing fluorescence correlation spectroscopy (FCS), we investigated the translational mobility of several biological macromolecules ranging from 17 kDa to 2.7 MDa. Polyethylene glycol and Ficoll polymers of different molecular masses were used in buffer solutions to mimic a crowded environment. The reduction in translational mobility of the biological tracer molecules was analyzed as a function of crowder volume fractions and was generally more pronounced in PEG as compared to Ficoll solutions. For several crowding conditions, we observed a molecular sieving effect, in which the diffusion coefficient of larger tracer molecules is reduced to a larger extent than predicted by the Stokes–Einstein relation. By employing a FRET-based biosensor, we also showed that a multiprotein complex is significantly compacted in the presence of macromolecular crowders. Importantly, with respect to sensor in vivo applications, ligand concentration determining sensors would need a crowding specific calibration in order to deliver correct cytosolic ligand concentration.
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000862852 7001_ $$0P:(DE-HGF)0$$aVaghefikia, Farzaneh$$b1
000862852 7001_ $$0P:(DE-HGF)0$$aAlbarghash, Alyazan$$b2
000862852 7001_ $$0P:(DE-Juel1)165927$$aHöfig, Henning$$b3
000862852 7001_ $$0P:(DE-HGF)0$$aKempe, Daryan$$b4
000862852 7001_ $$0P:(DE-HGF)0$$aWalter, Julia$$b5
000862852 7001_ $$0P:(DE-Juel1)165764$$aOtten, Julia$$b6
000862852 7001_ $$0P:(DE-HGF)0$$aPohl, Martina$$b7
000862852 7001_ $$0P:(DE-Juel1)131971$$aKatranidis, Alexandros$$b8
000862852 7001_ $$0P:(DE-Juel1)131034$$aWiegand, Simone$$b9
000862852 7001_ $$0P:(DE-Juel1)131961$$aFitter, Jörg$$b10$$eCorresponding author
000862852 773__ $$0PERI:(DE-600)2006039-7$$a10.1021/acs.jpcb.9b01239$$gp. acs.jpcb.9b01239$$n21$$p4477-4486$$tThe journal of physical chemistry <Washington, DC> / B B, Condensed matter, materials, surfaces, interfaces & biophysical$$v123$$x1520-5207$$y2019
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000862852 8564_ $$uhttps://juser.fz-juelich.de/record/862852/files/Manuscript_Crowding_JPC_B.pdf$$yPublished on 2019-05-06. Available in OpenAccess from 2020-05-06.
000862852 8564_ $$uhttps://juser.fz-juelich.de/record/862852/files/Manuscript_Crowding_JPC_B.pdf?subformat=pdfa$$xpdfa$$yPublished on 2019-05-06. Available in OpenAccess from 2020-05-06.
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