TY  - JOUR
AU  - Junker, Niklas O.
AU  - Vaghefikia, Farzaneh
AU  - Albarghash, Alyazan
AU  - Höfig, Henning
AU  - Kempe, Daryan
AU  - Walter, Julia
AU  - Otten, Julia
AU  - Pohl, Martina
AU  - Katranidis, Alexandros
AU  - Wiegand, Simone
AU  - Fitter, Jörg
TI  - Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules
JO  - The journal of physical chemistry  / B B, Condensed matter, materials, surfaces, interfaces & biophysical
VL  - 123
IS  - 21
SN  - 1520-5207
CY  - Washington, DC
PB  - Soc.66306
M1  - FZJ-2019-03051
SP  - 4477-4486
PY  - 2019
AB  - Effects of molecular crowding on structural and dynamical properties of biological macromolecules do depend on the concentration of crowding agents but also on the molecular mass and the structural compactness of the crowder molecules. By employing fluorescence correlation spectroscopy (FCS), we investigated the translational mobility of several biological macromolecules ranging from 17 kDa to 2.7 MDa. Polyethylene glycol and Ficoll polymers of different molecular masses were used in buffer solutions to mimic a crowded environment. The reduction in translational mobility of the biological tracer molecules was analyzed as a function of crowder volume fractions and was generally more pronounced in PEG as compared to Ficoll solutions. For several crowding conditions, we observed a molecular sieving effect, in which the diffusion coefficient of larger tracer molecules is reduced to a larger extent than predicted by the Stokes–Einstein relation. By employing a FRET-based biosensor, we also showed that a multiprotein complex is significantly compacted in the presence of macromolecular crowders. Importantly, with respect to sensor in vivo applications, ligand concentration determining sensors would need a crowding specific calibration in order to deliver correct cytosolic ligand concentration.
LB  - PUB:(DE-HGF)16
C6  - pmid:31059260
UR  - <Go to ISI:>//WOS:000470045700002
DO  - DOI:10.1021/acs.jpcb.9b01239
UR  - https://juser.fz-juelich.de/record/862852
ER  -