% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Junker:862852,
      author       = {Junker, Niklas O. and Vaghefikia, Farzaneh and Albarghash,
                      Alyazan and Höfig, Henning and Kempe, Daryan and Walter,
                      Julia and Otten, Julia and Pohl, Martina and Katranidis,
                      Alexandros and Wiegand, Simone and Fitter, Jörg},
      title        = {{I}mpact of {M}olecular {C}rowding on {T}ranslational
                      {M}obility and {C}onformational {P}roperties of {B}iological
                      {M}acromolecules},
      journal      = {The journal of physical chemistry / B B, Condensed matter,
                      materials, surfaces, interfaces $\&$ biophysical},
      volume       = {123},
      number       = {21},
      issn         = {1520-5207},
      address      = {Washington, DC},
      publisher    = {Soc.66306},
      reportid     = {FZJ-2019-03051},
      pages        = {4477-4486},
      year         = {2019},
      abstract     = {Effects of molecular crowding on structural and dynamical
                      properties of biological macromolecules do depend on the
                      concentration of crowding agents but also on the molecular
                      mass and the structural compactness of the crowder
                      molecules. By employing fluorescence correlation
                      spectroscopy (FCS), we investigated the translational
                      mobility of several biological macromolecules ranging from
                      17 kDa to 2.7 MDa. Polyethylene glycol and Ficoll polymers
                      of different molecular masses were used in buffer solutions
                      to mimic a crowded environment. The reduction in
                      translational mobility of the biological tracer molecules
                      was analyzed as a function of crowder volume fractions and
                      was generally more pronounced in PEG as compared to Ficoll
                      solutions. For several crowding conditions, we observed a
                      molecular sieving effect, in which the diffusion coefficient
                      of larger tracer molecules is reduced to a larger extent
                      than predicted by the Stokes–Einstein relation. By
                      employing a FRET-based biosensor, we also showed that a
                      multiprotein complex is significantly compacted in the
                      presence of macromolecular crowders. Importantly, with
                      respect to sensor in vivo applications, ligand concentration
                      determining sensors would need a crowding specific
                      calibration in order to deliver correct cytosolic ligand
                      concentration.},
      cin          = {ICS-5 / ICS-3 / IBG-1},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-5-20110106 / I:(DE-Juel1)ICS-3-20110106 /
                      I:(DE-Juel1)IBG-1-20101118},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31059260},
      UT           = {WOS:000470045700002},
      doi          = {10.1021/acs.jpcb.9b01239},
      url          = {https://juser.fz-juelich.de/record/862852},
}