% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Ahrens:862914,
      author       = {Ahrens, Dave and Rubner, Wolfgang and Springer, Ronald and
                      Hampe, Nico and Gehlen, Jenny and Magin, Thomas M. and
                      Hoffmann, Bernd and Merkel, Rudolf},
      title        = {{A} {C}ombined {AFM} and {L}ateral {S}tretch {D}evice
                      {E}nables {M}icroindentation {A}nalyses of {L}iving {C}ells
                      at {H}igh {S}trains},
      journal      = {Methods and protocols},
      volume       = {2},
      number       = {2},
      issn         = {2409-9279},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2019-03084},
      pages        = {43},
      year         = {2019},
      abstract     = {Mechanical characterization of living cells undergoing
                      substantial external strain promises insights into material
                      properties and functional principles of mechanically active
                      tissues. However, due to the high strains that occur in
                      physiological situations (up to $50\%)$ and the complex
                      structure of living cells, suitable experimental techniques
                      are rare. In this study, we introduce a new system composed
                      of an atomic force microscope (AFM), a cell stretching
                      system based on elastomeric substrates, and light
                      microscopy. With this system, we investigated the influence
                      of mechanical stretch on monolayers of keratinocytes. In
                      repeated indentations at the same location on one particular
                      cell, we found significant stiffening at $25\%$ and $50\%$
                      strain amplitude. To study the contribution of intermediate
                      filaments, we used a mutant keratinocyte cell line devoid of
                      all keratins. For those cells, we found a softening in
                      comparison to the wild type, which was even more pronounced
                      at higher strain amplitudes.},
      cin          = {ICS-7},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553) / 552 -
                      Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-553 / G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31164622},
      UT           = {WOS:000698975100018},
      doi          = {10.3390/mps2020043},
      url          = {https://juser.fz-juelich.de/record/862914},
}