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@ARTICLE{Khan:863297,
      author       = {Khan, Imran and Gratz, Regina and Denezhkin, Polina and
                      Schott-Verdugo, Stephan N. and Angrand, Kalina and Genders,
                      Lara and Basgaran, Rubek Merina and Fink-Straube, Claudia
                      and Brumbarova, Tzvetina and Gohlke, Holger and Bauer, Petra
                      and Ivanov, Rumen},
      title        = {{C}alcium-promoted interaction between the {C}2-domain
                      protein {EHB}1 and metal transporter {IRT}1 inhibits
                      {A}rabidopsis iron acquisition},
      journal      = {Plant physiology},
      volume       = {180},
      number       = {3},
      issn         = {1532-2548},
      address      = {Rockville, Md.},
      publisher    = {Soc.},
      reportid     = {FZJ-2019-03384},
      pages        = {1564-1581},
      year         = {2019},
      abstract     = {Iron is a key transition element in the biosphere and is
                      crucial for living organisms, although its cellular excess
                      can be deleterious. Maintaining the balance of optimal iron
                      availability in the model plant Arabidopsis (Arabidopsis
                      thaliana) requires the precise operation of iron import
                      through the principal iron transporter IRON-REGULATED
                      TRANSPORTER1 (IRT1). Targeted inhibition of IRT1 can prevent
                      oxidative stress, thus promoting plant survival. Here, we
                      report the identification of an IRT1 inhibitor, namely the
                      C2 domain-containing peripheral membrane protein ENHANCED
                      BENDING1 (EHB1). EHB1 interacts with the cytoplasmically
                      exposed variable region of IRT1, and we demonstrate that
                      this interaction is greatly promoted by the presence of
                      calcium. We found that EHB1 binds lipids characteristic of
                      the plasma membrane, and the interaction between EHB1 and
                      plant membranes is calcium dependent. Molecular simulations
                      showed that EHB1 membrane binding is a two-step process that
                      precedes the interaction between EHB1 and IRT1. Genetic and
                      physiological analyses indicated that EHB1 acts as a
                      negative regulator of iron acquisition. The presence of EHB1
                      prevented the IRT1-mediated complementation of
                      iron-deficient fet3fet4 yeast. Our data suggest that EHB1
                      acts as a direct inhibitor of IRT1-mediated iron import into
                      the cell. These findings represent a major step in
                      understanding plant iron acquisition, a process that
                      underlies the primary production of bioavailable iron for
                      land ecosystems.},
      cin          = {NIC / ICS-6 / JSC},
      ddc          = {580},
      cid          = {I:(DE-Juel1)NIC-20090406 / I:(DE-Juel1)ICS-6-20110106 /
                      I:(DE-Juel1)JSC-20090406},
      pnm          = {511 - Computational Science and Mathematical Methods
                      (POF3-511) / Forschergruppe Gohlke $(hkf7_20170501)$},
      pid          = {G:(DE-HGF)POF3-511 / $G:(DE-Juel1)hkf7_20170501$},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31040174},
      UT           = {WOS:000473274000027},
      doi          = {10.1104/pp.19.00163},
      url          = {https://juser.fz-juelich.de/record/863297},
}