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@ARTICLE{Endepols:863617,
author = {Endepols, Heike and Morgenroth, Agnieszka and Zlatopolskiy,
Boris D. and Krapf, Philipp and Zischler, Johannes and
Richarz, Raphael and Muñoz Vásquez, Sergio and Neumaier,
Bernd and Mottaghy, Felix M.},
title = {{P}eripheral ganglia in healthy rats as target structures
for the evaluation of {PSMA} imaging agents},
journal = {BMC cancer},
volume = {19},
number = {1},
issn = {1471-2407},
address = {Heidelberg},
publisher = {Springer},
reportid = {FZJ-2019-03629},
pages = {633},
year = {2019},
abstract = {BACKGROUND:The recent implementation of PET with prostate
specific membrane antigen (PSMA)-specific radiotracers into
the clinical practice has resulted in the significant
improvement of accuracy in the detection of prostate
carcinoma (PCa). PSMA-expression in ganglia has been
regarded as an important pitfall in prostate carcinoma-PET
diagnostics but has not found any practical use for
diagnosis or therapy.METHODS:We explored this phenomenon and
demonstrated the applicability of peripheral ganglia in
healthy rats as surrogates for small PSMA positive lesions
for the preclinical evaluation of diagnostic PCa PET probes.
Healthy rats were measured with PET/CT using the tracers
[18F]DCFPyL, [Al18F]PSMA-11 and [68Ga]PSMA-11. Sections of
ganglia were stained with an anti-PSMA antibody. [18F]DCFPyL
uptake in ganglia was compared to that in LNCaP tumor
xenografts in mice.RESULTS:Whereas [18F]DCFPyL and
[68Ga]PSMA-11 were stable in vivo and accumulated in
peripheral ganglia, [Al18F]PSMA-11 suffered from fast in
vivo deflourination resulting in high bone uptake.
Ganglionic PSMA expression was confirmed by
immunohistochemistry. [18F]DCFPyL uptake and signal-to-noise
ratio in the superior cervical ganglion was not
significantly different from LNCaP
xenografts.CONCLUSIONS:Our results demonstrated the
non-inferiority of the novel model compared to
conventionally used tumor xenografts in immune compromised
rodents with regard to reproducibility and stability of the
PSMA signal. Furthermore, the model involves less expense
and efforts while it is permanently available and avoids
tumor-growth associated animal morbidity and distress. To
the best of our knowledge, this is the first tumor-free
model suitable for the in vivo evaluation of tumor imaging
agents.},
cin = {INM-5},
ddc = {610},
cid = {I:(DE-Juel1)INM-5-20090406},
pnm = {573 - Neuroimaging (POF3-573)},
pid = {G:(DE-HGF)POF3-573},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31242896},
UT = {WOS:000473007000002},
doi = {10.1186/s12885-019-5841-8},
url = {https://juser.fz-juelich.de/record/863617},
}