TY  - JOUR
AU  - Kolašinac, Rejhana
AU  - Jaksch, Sebastian
AU  - Dreissen, Georg
AU  - Braeutigam, Andrea
AU  - Merkel, Rudolf
AU  - Csiszar, Agnes
TI  - Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells
JO  - Nanomaterials
VL  - 9
IS  - 7
SN  - 2079-4991
CY  - Basel
PB  - MDPI
M1  - FZJ-2019-03877
SP  - 1025 -
PY  - 2019
AB  - Lipid-based nanoparticles, also called vesicles or liposomes, can be used as carriers for drugs or many types of biological macromolecules, including DNA and proteins. Efficiency and speed of cargo delivery are especially high for carrier vesicles that fuse with the cellular plasma membrane. This occurs for lipid mixture containing equal amounts of the cationic lipid DOTAP and a neutral lipid with an additional few percents of an aromatic substance. The fusion ability of such particles depends on lipid composition with phosphoethanolamine (PE) lipids favoring fusion and phosphatidyl-choline (PC) lipids endocytosis. Here, we examined the effects of temperature, ionic strength, osmolality, and pH on fusion efficiency of cationic liposomes with Chinese hamster ovary (CHO) cells. The phase state of liposomes was analyzed by small angle neutron scattering (SANS). Our results showed that PC containing lipid membranes were organized in the lamellar phase. Here, fusion efficiency depended on buffer conditions and remained vanishingly small at physiological conditions. In contrast, SANS indicated the coexistence of very small (~50 nm) objects with larger, most likely lamellar structures for PE containing lipid particles. The fusion of such particles to cell membranes occurred with very high efficiency at all buffer conditions. We hypothesize that the altered phase state resulted in a highly reduced energetic barrier against fusion
LB  - PUB:(DE-HGF)16
C6  - pmid:31319557
UR  - <Go to ISI:>//WOS:000478992600113
DO  - DOI:10.3390/nano9071025
UR  - https://juser.fz-juelich.de/record/863912
ER  -