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@ARTICLE{Kolainac:863912,
      author       = {Kolašinac, Rejhana and Jaksch, Sebastian and Dreissen,
                      Georg and Braeutigam, Andrea and Merkel, Rudolf and Csiszar,
                      Agnes},
      title        = {{I}nfluence of {E}nvironmental {C}onditions on the {F}usion
                      of {C}ationic {L}iposomes with {L}iving {M}ammalian {C}ells},
      journal      = {Nanomaterials},
      volume       = {9},
      number       = {7},
      issn         = {2079-4991},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2019-03877},
      pages        = {1025 -},
      year         = {2019},
      abstract     = {Lipid-based nanoparticles, also called vesicles or
                      liposomes, can be used as carriers for drugs or many types
                      of biological macromolecules, including DNA and proteins.
                      Efficiency and speed of cargo delivery are especially high
                      for carrier vesicles that fuse with the cellular plasma
                      membrane. This occurs for lipid mixture containing equal
                      amounts of the cationic lipid DOTAP and a neutral lipid with
                      an additional few percents of an aromatic substance. The
                      fusion ability of such particles depends on lipid
                      composition with phosphoethanolamine (PE) lipids favoring
                      fusion and phosphatidyl-choline (PC) lipids endocytosis.
                      Here, we examined the effects of temperature, ionic
                      strength, osmolality, and pH on fusion efficiency of
                      cationic liposomes with Chinese hamster ovary (CHO) cells.
                      The phase state of liposomes was analyzed by small angle
                      neutron scattering (SANS). Our results showed that PC
                      containing lipid membranes were organized in the lamellar
                      phase. Here, fusion efficiency depended on buffer conditions
                      and remained vanishingly small at physiological conditions.
                      In contrast, SANS indicated the coexistence of very small
                      (~50 nm) objects with larger, most likely lamellar
                      structures for PE containing lipid particles. The fusion of
                      such particles to cell membranes occurred with very high
                      efficiency at all buffer conditions. We hypothesize that the
                      altered phase state resulted in a highly reduced energetic
                      barrier against fusion},
      cin          = {ICS-7 / JCNS-FRM-II},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-7-20110106 /
                      I:(DE-Juel1)JCNS-FRM-II-20110218},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      experiment   = {EXP:(DE-MLZ)KWS2-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31319557},
      UT           = {WOS:000478992600113},
      doi          = {10.3390/nano9071025},
      url          = {https://juser.fz-juelich.de/record/863912},
}