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@ARTICLE{Tomasova:863917,
      author       = {Tomasova, Lea and Guttenberg, Zeno and Hoffmann, Bernd and
                      Merkel, Rudolf},
      title        = {{A}dvanced 2{D}/3{D} cell migration assay for faster
                      evaluation of chemotaxis of slow-moving cells},
      journal      = {PLOS ONE},
      volume       = {14},
      number       = {7},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {FZJ-2019-03880},
      pages        = {e0219708 -},
      year         = {2019},
      abstract     = {Considering the essential role of chemotaxis of adherent,
                      slow-moving cells in processes such as tumor metastasis or
                      wound healing, a detailed understanding of the mechanisms
                      and cues that direct migration of cells through tissues is
                      highly desirable. The state-of-the-art chemotaxis
                      instruments (e.g. microfluidic-based devices, bridge assays)
                      can generate well-defined, long-term stable chemical
                      gradients, crucial for quantitative investigation of
                      chemotaxis in slow-moving cells. However, the majority of
                      chemotaxis tools are designed for the purpose of an
                      in-depth, but labor-intensive analysis of migratory behavior
                      of single cells. This is rather inefficient for applications
                      requiring higher experimental throughput, as it is the case
                      of e.g. clinical examinations, chemoattractant screening or
                      studies of the chemotaxis-related signaling pathways based
                      on subcellular perturbations. Here, we present an advanced
                      migration assay for accelerated and facilitated evaluation
                      of the chemotactic response of slow-moving cells. The
                      revised chemotaxis chamber contains a hydrogel
                      microstructure–the migration arena, designed to enable
                      identification of chemotactic behavior of a cell population
                      in respect to the end-point of the experiment. At the same
                      time, the assay in form of a microscopy slide enables direct
                      visualization of the cells in either 2D or 3D environment,
                      and provides a stable and linear gradient of
                      chemoattractant. We demonstrate the correctness of the assay
                      on the model study of HT-1080 chemotaxis in 3D and on 2D
                      surface. Finally, we apply the migration arena chemotaxis
                      assay to screen for a chemoattractant of primary
                      keratinocytes, cells that play a major role in wound
                      healing, being responsible for skin re-epithelialization and
                      a successful wound closure. In direction of new therapeutic
                      strategies to promote wound repair, we identified the
                      chemotactic activity of the epithelial growth factor
                      receptor (EGFR) ligands EGF and TGFα (transforming growth
                      factor α).},
      cin          = {ICS-7},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31314801},
      UT           = {WOS:000482331900039},
      doi          = {10.1371/journal.pone.0219708},
      url          = {https://juser.fz-juelich.de/record/863917},
}