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000864225 1001_ $$0P:(DE-HGF)0$$aBovdilova, Anastasiia$$b0
000864225 245__ $$aPosttranslational modification of the NADP-malic enzyme involved in C4 photosynthesis fine-tunes the enzymatic activity during the day
000864225 260__ $$aRockville, Md.$$bSoc.$$c2019
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000864225 520__ $$aEvolution of the C4 photosynthetic pathway involved in some cases recruitment of housekeeping proteins through gene duplication and their further neofunctionalization. NADP-malic enzyme (ME), the most widespread C4 decarboxylase, has increased its catalytic efficiency and acquired regulatory properties that allowed it to participate in the C4 pathway. Here, we show that regulation of maize C4-NADP-ME activity is much more elaborated than until now indicated. Using mass spectrometry, we identified phosphorylation of the serine 419 (S419) of C4-NADP-ME in protein extracts of maize leaves. The phosphorylation event increases after the light turns on, with a peak at ZT2. Phosphorylation of ZmC4-NADP-ME drastically decreases its activity as shown by the low residual activity of the recombinant phosphomimetic mutant. Analysis of the crystal structure of C4-NADP-ME indicated that S419 is involved in the binding of NADP at the active site. Molecular dynamics simulations and effective binding energy computations indicate a less favorable binding of the cofactor NADP in the phosphomimetic and the phosphorylated variants. We propose that phosphorylation of ZmC4-NADP-ME at S419 during the first hours in the light is a cellular mechanism to fine-tune the enzymatic activity to coordinate the carbon concentration mechanism with the CO2 fixation rate, most probably to avoid CO2 leakiness from bundle sheath cells.
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000864225 7001_ $$0P:(DE-HGF)0$$aAlexandre, Bruno M.$$b1
000864225 7001_ $$0P:(DE-HGF)0$$aHöppner, Astrid$$b2
000864225 7001_ $$0P:(DE-HGF)0$$aMatias Luís, Inês$$b3
000864225 7001_ $$0P:(DE-HGF)0$$aAlvarez, Clarisa E$$b4
000864225 7001_ $$0P:(DE-HGF)0$$aBickel, David$$b5
000864225 7001_ $$0P:(DE-Juel1)172663$$aGohlke, Holger$$b6
000864225 7001_ $$0P:(DE-Juel1)162438$$aDecker, Christina$$b7
000864225 7001_ $$0P:(DE-Juel1)162443$$aNagel-Steger, Luitgard$$b8
000864225 7001_ $$0P:(DE-HGF)0$$aAlseekh, Saleh$$b9
000864225 7001_ $$0P:(DE-HGF)0$$aFernie, Alisdair R.$$b10
000864225 7001_ $$0P:(DE-HGF)0$$aDrincovich, Maria F$$b11
000864225 7001_ $$0P:(DE-HGF)0$$aAbreu, Isabel A.$$b12
000864225 7001_ $$0P:(DE-HGF)0$$aMaurino, Veronica G$$b13$$eCorresponding author
000864225 773__ $$0PERI:(DE-600)2004373-9$$a10.1105/tpc.19.00406$$gp. tpc.00406.2019 -$$n10$$p2525-2539$$tThe plant cell$$v31$$x1040-4651$$y2019
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