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@ARTICLE{Wang:864990,
author = {Wang, Liming and Kuchendorf, Christina and Willbold,
Sabine},
title = {{D}etermination of individual chain length and chain-length
distribution of polyphosphates in microalgae by
31{P}-{DOSY}-{NMR}},
journal = {Algal Research},
volume = {43},
issn = {2211-9264},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {FZJ-2019-04567},
pages = {101631 -101634},
year = {2019},
abstract = {Due to its ecological and biotechnological relevance,
polyphosphate in microalgae is currently the focus of
intense research. Numerous biological functions are
performed by or dependent on polyphosphate, and they depend,
among other factors, on its chain length. Chain length
determination is important for understanding polyphosphate
metabolism and for maximizing intracellular polyphosphate
abundance per unit weight of biomass. 31P-DOSY NMR virtually
separates various polyphosphate polymers in a mixture based
on different translational diffusion coefficients. The
diffusion coefficient of a polyphosphate molecule correlates
with its molecular weight, enabling determination of
individual chain lengths. Moreover, the polydispersity index
can also be uniquely determined by DOSY as a measure of the
overall chain-length distribution of polyphosphates. By
contrast, conventional 31P NMR is only able to estimate the
average chain length of the entire polyphosphate pool.
Therefore, DOSY provides the opportunity to deepen our
insight into polyphosphate metabolism and dynamics in algal
biomass.},
cin = {ZEA-3 / IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)ZEA-3-20090406 / I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000489307800010},
doi = {10.1016/j.algal.2019.101631},
url = {https://juser.fz-juelich.de/record/864990},
}