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@INPROCEEDINGS{Matsubara:865232,
      author       = {Matsubara, Shizue and Thiele, Björn and Banh, Anh and
                      Chlubek and Hombach, Thomas and Neuwohner, Andrea and
                      Janzik, Ingar and Jahnke, Siegfried and Kleist, Einhard},
      title        = {{T}oward understanding the regulation of carotenoid
                      turnover in plants},
      reportid     = {FZJ-2019-04763},
      year         = {2019},
      abstract     = {Progress in plant carotenoid research has brought the
                      scientific community genetic and molecular tools to
                      manipulate carotenoid composition and accumulation in model
                      plants and crops. However, the outcome of such manipulation
                      often remains unpredictable due to many “unknowns” in
                      feedback regulation, protein modification and interaction,
                      or compartmentalization. Unravelling the mechanisms that
                      control flux, partitioning and sequestration in carotenoid
                      biosynthesis and degradation pathways necessitates, among
                      other things, turnover analysis of pathway intermediates and
                      products. Isotopic labelling has been a powerful tool for
                      investigation of metabolic pathways in cells and plants.
                      Photosynthetic CO2 fixation enables convenient incorporation
                      of carbon isotopes in various metabolites under various
                      conditions. While incorporation can be observed within
                      seconds to minutes in and around primary metabolism, it can
                      take hours to days for specialized metabolism, including
                      that of carotenoids and apocarotenoids in photosynthetic and
                      non-photosynthetic organs. Despite high sensitivity of
                      detection, radioactive 14C is difficult to quantify at the
                      molecular level and experiments inevitably generate
                      radioactive waste. Here, we present whole-plant 13CO2
                      labelling systems combined with LC-MS and FTICR-MS analysis
                      to study carotenoid turnover. Two experiments were conducted
                      to test the applicability of the method. In the first
                      experiment, a walk-in climate chamber was used to obtain
                      13C-labelled peppermint plants. Subsequently, changes in
                      carotenoid isotopologue composition were monitored in leaves
                      of these plants in ambient air. In the second experiment,
                      Arabidopsis plants grown in ambient air were transferred to
                      a small 13CO2 labelling chamber to analyze 13C incorporation
                      in leaf pigments for up to seven days. The mass spectra of
                      carotenoids obtained from the two experiments showed similar
                      patterns of isotopologue peaks, indicating that the method
                      is compatible with pulse-chase labelling experiments in both
                      directions (13CO2/12CO2 and 12CO2/13CO2). This approach
                      opens up new possibilities to elucidate the regulation of
                      carotenoid metabolism in plants.},
      month         = {Nov},
      date          = {2019-11-25},
      organization  = {International Conference on Carotenoid
                       Research, Lemesos (Cyprus), 25 Nov 2019
                       - 28 Nov 2019},
      subtyp        = {Other},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/865232},
}