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@ARTICLE{Weis:865235,
      author       = {Weis, Felix and Beckers, Maximilian and Hocht, Iris and
                      Sachse, Carsten},
      title        = {{E}lucidation of the viral disassembly switch of tobacco
                      mosaic virus},
      journal      = {EMBO reports},
      volume       = {20},
      number       = {11},
      issn         = {1469-3178},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2019-04766},
      pages        = {e48451},
      year         = {2019},
      abstract     = {Stable capsid structures of viruses protect viral RNA while
                      they also require controlled disassembly for releasing the
                      viral genome in the host cell. A detailed understanding of
                      viral disassembly processes and the involved structural
                      switches is still lacking. This process has been extensively
                      studied using tobacco mosaic virus (TMV), and carboxylate
                      interactions are assumed to play a critical part in this
                      process. Here, we present two cryo‐EM structures of the
                      helical TMV assembly at 2.0 and 1.9 Å resolution in
                      conditions of high Ca2+ concentration at low pH and in
                      water. Based on our atomic models, we identify the
                      conformational details of the disassembly switch mechanism:
                      In high Ca2+/acidic pH environment, the virion is stabilized
                      between neighboring subunits through carboxyl groups E95 and
                      E97 in close proximity to a Ca2+ binding site that is shared
                      between two subunits. Upon increase in pH and lower Ca2+
                      levels, mutual repulsion of the E95/E97 pair and Ca2+
                      removal destabilize the network of interactions between
                      adjacent subunits at lower radius and release the switch for
                      viral disassembly.},
      cin          = {ER-C-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31535454},
      UT           = {WOS:000487086400001},
      doi          = {10.15252/embr.201948451},
      url          = {https://juser.fz-juelich.de/record/865235},
}