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@ARTICLE{Hondrich:865344,
      author       = {Hondrich, Timm J. J. and Deußen, Oliver and Grannemann,
                      Caroline and Brinkmann, Dominik and Offenhäusser, Andreas},
      title        = {{I}mprovements of microcontact printing for micropatterned
                      cell growth by contrast enhancements},
      journal      = {Micromachines},
      volume       = {10},
      number       = {10},
      issn         = {2072-666X},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2019-04843},
      pages        = {659 -},
      year         = {2019},
      abstract     = {Patterned neuronal cell cultures are important tools for
                      investigating neuronal signal integration, network function,
                      and cell–substrate interactions. Because of the variable
                      nature of neuronal cells, the widely used coating method of
                      microcontact printing is in constant need of improvements
                      and adaptations depending on the pattern, cell type, and
                      coating solutions available for a certain experimental
                      system. In this work, we report on three approaches to
                      modify microcontact printing on borosilicate glass surfaces,
                      which we evaluate with contact angle measurements and by
                      determining the quality of patterned neuronal growth.
                      Although background toxification with manganese salt does
                      not result in the desired pattern enhancement, a simple heat
                      treatment of the glass substrates leads to improved
                      background hydrophobicity and therefore neuronal patterning.
                      Thirdly, we extended a microcontact printing process based
                      on covalently linking the glass surface and the coating
                      molecule via an epoxysilane. This extension is an additional
                      hydrophobization step with dodecylamine. We demonstrate that
                      shelf life of the silanized glass is at least 22 weeks,
                      leading to consistently reliable neuronal patterning by
                      microcontact printing. Thus, we compared three practical
                      additions to microcontact printing, two of which can easily
                      be implemented into a workflow for the investigation of
                      patterned neuronal networks},
      cin          = {ICS-8},
      ddc          = {620},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31574944},
      UT           = {WOS:000494485000033},
      doi          = {10.3390/mi10100659},
      url          = {https://juser.fz-juelich.de/record/865344},
}