000865990 001__ 865990
000865990 005__ 20220930130221.0
000865990 0247_ $$2doi$$a10.3390/ijms20184608
000865990 0247_ $$2ISSN$$a1422-0067
000865990 0247_ $$2ISSN$$a1661-6596
000865990 0247_ $$2Handle$$a2128/23160
000865990 0247_ $$2pmid$$apmid:31533368
000865990 0247_ $$2WOS$$aWOS:000489100500287
000865990 037__ $$aFZJ-2019-05251
000865990 082__ $$a540
000865990 1001_ $$0P:(DE-Juel1)167181$$aHilgers, Fabienne$$b0
000865990 245__ $$aGenetically encoded photosensitizers as light-triggered antimicrobial agents
000865990 260__ $$aBasel$$bMolecular Diversity Preservation International$$c2019
000865990 3367_ $$2DRIVER$$aarticle
000865990 3367_ $$2DataCite$$aOutput Types/Journal article
000865990 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1572437056_20861
000865990 3367_ $$2BibTeX$$aARTICLE
000865990 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000865990 3367_ $$00$$2EndNote$$aJournal Article
000865990 520__ $$aDiseases caused by multi-drug resistant pathogens have become a global concern. Therefore, new approaches suitable for treating these bacteria are urgently needed. In this study, we analyzed genetically encoded photosensitizers (PS) related to the green fluorescent protein (GFP) or light-oxygen-voltage (LOV) photoreceptors for their exogenous applicability as light-triggered antimicrobial agents. Depending on their specific photophysical properties and photochemistry, these PSs can produce different toxic ROS (reactive oxygen species) such as O2•− and H2O2 via type-I, as well as 1O2 via type-II reaction in response to light. By using cell viability assays and microfluidics, we could demonstrate differences in the intracellular and extracellular phototoxicity of the applied PS. While intracellular expression and exogenous supply of GFP-related PSs resulted in a slow inactivation of E. coli and pathogenic Gram-negative and Gram-positive bacteria, illumination of LOV-based PSs such as the singlet oxygen photosensitizing protein SOPP3 resulted in a fast and homogeneous killing of these microbes. Furthermore, our data indicate that the ROS type and yield as well as the localization of the applied PS protein can strongly influence the antibacterial spectrum and efficacy. These findings open up new opportunities for photodynamic inactivation of pathogenic bacteria
000865990 536__ $$0G:(DE-HGF)POF3-581$$a581 - Biotechnology (POF3-581)$$cPOF3-581$$fPOF III$$x0
000865990 588__ $$aDataset connected to CrossRef
000865990 7001_ $$0P:(DE-Juel1)174200$$aBitzenhofer, Nora Lisa$$b1
000865990 7001_ $$0P:(DE-Juel1)174544$$aAckermann, Yannic$$b2
000865990 7001_ $$0P:(DE-Juel1)172901$$aBurmeister, Alina$$b3
000865990 7001_ $$0P:(DE-Juel1)143612$$aGrünberger, Alexander$$b4$$ufzj
000865990 7001_ $$0P:(DE-Juel1)131457$$aJaeger, Karl-Erich$$b5
000865990 7001_ $$0P:(DE-Juel1)131426$$aDrepper, Thomas$$b6$$eCorresponding author
000865990 773__ $$0PERI:(DE-600)2019364-6$$a10.3390/ijms20184608$$gVol. 20, no. 18, p. 4608 -$$n18$$p4608 -$$tInternational journal of molecular sciences$$v20$$x1422-0067$$y2019
000865990 8564_ $$uhttps://juser.fz-juelich.de/record/865990/files/Invoice_MDPI_ijms-576476.pdf
000865990 8564_ $$uhttps://juser.fz-juelich.de/record/865990/files/Invoice_MDPI_ijms-576476.pdf?subformat=pdfa$$xpdfa
000865990 8564_ $$uhttps://juser.fz-juelich.de/record/865990/files/ijms-20-04608.pdf$$yOpenAccess
000865990 8564_ $$uhttps://juser.fz-juelich.de/record/865990/files/ijms-20-04608.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000865990 8767_ $$8ijms-57647$$92019-09-14$$d2019-09-16$$eAPC$$jZahlung erfolgt$$zFZJ-2019-04711
000865990 909CO $$ooai:juser.fz-juelich.de:865990$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire$$pdnbdelivery
000865990 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)167181$$aForschungszentrum Jülich$$b0$$kFZJ
000865990 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)174200$$aForschungszentrum Jülich$$b1$$kFZJ
000865990 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)172901$$aForschungszentrum Jülich$$b3$$kFZJ
000865990 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)143612$$aForschungszentrum Jülich$$b4$$kFZJ
000865990 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131457$$aForschungszentrum Jülich$$b5$$kFZJ
000865990 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131426$$aForschungszentrum Jülich$$b6$$kFZJ
000865990 9131_ $$0G:(DE-HGF)POF3-581$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vBiotechnology$$x0
000865990 9141_ $$y2019
000865990 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000865990 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000865990 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000865990 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bINT J MOL SCI : 2017
000865990 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000865990 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000865990 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000865990 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000865990 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000865990 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000865990 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000865990 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000865990 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000865990 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000865990 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000865990 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000865990 9201_ $$0I:(DE-Juel1)IMET-20090612$$kIMET$$lInstitut für Molekulare Enzymtechnologie (HHUD)$$x0
000865990 9201_ $$0I:(DE-Juel1)IBG-1-20101118$$kIBG-1$$lBiotechnologie$$x1
000865990 9801_ $$aFullTexts
000865990 980__ $$ajournal
000865990 980__ $$aVDB
000865990 980__ $$aI:(DE-Juel1)IMET-20090612
000865990 980__ $$aI:(DE-Juel1)IBG-1-20101118
000865990 980__ $$aUNRESTRICTED
000865990 980__ $$aAPC